The effects of fetal adrenalectomy at 110 days gestational age on AVP and CRH mRNA expression in the hypothalamic paraventricular nucleus of the ovine fetus

Brain Research. Developmental Brain Research
N UnnoP W Nathanielsz

Abstract

AVP and CRH produced in the parvocellular neurons of the paraventricular nucleus (PVN) have both been implicated in the regulation of anterior pituitary ACTH synthesis and secretion. In sheep, fetal ACTH secretion increases around 120 days gestational age (dGA). Little is known about adrenal regulation of AVP and CRH immediately prior to this critical period. We investigated the effects of adrenalectomy and subsequent cortisol (F) administration on PVN AVP and CRH mRNA in the fetal sheep PVN at 109-125 dGA. At 109-113 dGA, fetal sheep adrenals were removed (ADX)(n = 8); or sham surgery performed (CONT)(n = 4). From day 6 post ADX, maternal plasma cortisol and fetal plasma ACTH and cortisol levels were determined daily by radioimmunoassays. From day 7 post ADX, cortisol (4 micrograms/min) was continuously infused intravenously to four ADX fetuses (ADX + F). Fetal hypothalami were collected at 123-125 dGA, and studied by in-situ hybridization and quantitative autoradiography for AVP and CRH mRNA. Plasma cortisol levels remained low in CONT and ADX fetuses (< 4.9 ng/ml), while during cortisol infusion to ADX + F fetuses, plasma F increased to 16.4 +/- 2.2 and 22.3 +/- 3.2 ng/ml (mean +/- S.E.M.) on day 10 and 13, respectively. Pla...Continue Reading

References

Sep 1, 1991·American Journal of Obstetrics and Gynecology·T J McDonald, P W Nathanielsz
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Citations

Jun 11, 1998·European Journal of Obstetrics, Gynecology, and Reproductive Biology·P W Nathanielsz
May 9, 2014·Journal of Neuropathology and Experimental Neurology·Daniela VirgintinoBarbara S Stonestreet
Feb 14, 2006·The Journal of Physiology·Amita KapoorStephen G Matthews
May 24, 2005·Reproduction, Fertility, and Development·Nitin P RonBarbara S Stonestreet
Apr 13, 1999·The American Journal of Physiology·M A AnwarP W Nathanielsz

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