The effects of fibroblast growth factor-2 on human neonatal calvaria osteoblastic cells are differentiation stage specific

Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research
F DebiaisP J Marie

Abstract

Fibroblast growth factors (FGFs) appear to play an important role in human cranial osteogenesis. We therefore investigated the effects of recombinant human FGF-2 (rhFGF-2) on human calvaria (HC) osteoblastic cells. Immunocytochemical analysis showed that confluent HC cells express both FGF receptors -1 and -2. In short-term culture, rhFGF-2 (0.1-100 ng/ml, 2-5 days) increased HC cell growth and decreased alkaline phosphatase (ALP) activity and type I collagen (ColI) synthesis, as evaluated by P1CP levels. When HC cells were induced to differentiate in long-term culture in the presence of 50 microg/ml ascorbic acid and 3 mM phosphate, HC cells initially proliferated, then ALP activity and ColI synthesis decreased and calcium content in the extracellular matrix increased. Continuous treatment with rhFGF-2 (50 ng/ml) for 1-28 days, or a transient rhFGF-2 treatment for 1-7 days, slightly increased DNA synthesis at 7 days, whereas a late treatment for 8-28 days had no effect on cell growth. The continuous and transient treatments with rhFGF-2 decreased ALP activity, ColI synthesis, and matrix mineralization. This was associated with a transient fall in osteocalcin (OC) production at 7 days. In contrast, the late rhFGF-2 treatment fo...Continue Reading

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Citations

Apr 9, 2008·The Journal of Biological Chemistry·Mikihito KajiyaHidemi Kurihara
May 29, 2008·Molecular and Cellular Biology·Davide AmbrosettiClaudio Basilico
Jan 6, 2010·The Journal of Biological Chemistry·Siddharth R VoraPhilip C Trackman
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