PMID: 145797Sep 1, 1977Paper

The effects of fractions (chalones) obtained from lymphoid organs on lymphocyte proliferation in vitro

Agents and Actions
P C HiestandH F Stähelin

Abstract

Aqueous extracts of lymphoid organs were prepared and fractionated by means of gel filtration, ion exchange chromatography, and isoelectric focusing. A protein-containing fraction with a molecular weight of approximately 80,000--90,000 and isoelectric points of 7.6 and 5.3--6.2 was isolated and shown to inhibit reproducibly both thymidine incorporation and proliferation of concanavalin A-stimulated mouse spleen lymphocytes in vitro. This effect appeared specific since proliferation of mastocytoma P-815 and leukemia L-1210 cells remained unaffected. A small molecular weight fraction (500 to 10,000 daltons) was also found to inhibit lymphocyte proliferation in vitro but was without apparent specificity for cell type.

References

Feb 1, 1975·Life Sciences·W S Bullough
Jul 1, 1975·Experimental Cell Research·A AttallahJ C Houck
Nov 18, 1976·Biochimica Et Biophysica Acta·M LenfantC Tempête
Sep 1, 1973·Lancet·T D Hunter
Apr 1, 1971·Agents and Actions·W S Bullough
Sep 1, 1974·Cell·I C Henderson, D M Livingston
Jan 1, 1972·Transplantation Reviews·M Greaves, G Janossy
Sep 17, 1971·Science·J C HouckS Leikin
Mar 1, 1960·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·W S Bullough, E B LAURENCE
Jan 1, 1962·Medicina Experimentalis. International Journal of Experimental Medicine·H STAHELIN
Jan 1, 1962·Clinica Chimica Acta; International Journal of Clinical Chemistry·C J MULLER, C PIK

Citations

Jan 1, 1995·Folia Microbiologica·Z Rehácek
Jan 1, 1994·Artificial Cells, Blood Substitutes, and Immobilization Biotechnology·L BrasileC Haisch

Related Concepts

Metazoa
Bos indicus
Cell Division Phases
Molecular Sieve Chromatography
Ion-Exchange Chromatography Procedure
Growth Inhibitors
Isoelectric Focusing
Lymphoid Cells
Lymphoid Tissue
Mice, Inbred CBA

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