The effects of halothane on single human neuronal L-type calcium channels

Anesthesia and Analgesia
I M NikonorovE Recio-Pinto

Abstract

We investigated halothane's effects on the function of L-type Ca2+ channels in a human neuronal cell line, SH-SY5Y, by using the cell-attached patch voltage clamp configuration and Ba2+ as the charge carrier. In multiple-channel patches, halothane decreased the peak and persistent Ba2+ currents, accelerated the rate of inactivation, and slowed the rate of activation. Single-channel analysis showed that halothane (0.14-1.26 mM) increased the latency time for the first channel opening, increased the lifetime of nonconducting events, increased the proportion of short-lived open events, decreased the lifetime of the two open populations, and increased the percentage of current traces without channel activity. All of the observed halothane effects contribute to the halothane-induced decrease in macroscopic Ba2+ currents. The halothane concentration producing 50% reduction (IC50) of the peak Ba2+ current was 0.80 mM (approximately 1.9 hypothetical minimum alveolar anesthetic concentration [H-MAC] at 28 degrees C) and of the persistent Ba2+ current was 0.69 mM (approximately 1.7 H-MAC). The halothane effects did not always occur together, and the Hill slope of 1.6 suggested the presence of more than one interaction site or of more tha...Continue Reading

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Citations

Feb 2, 2002·Canadian Journal of Anaesthesia = Journal Canadien D'anesthésie·Michiaki Yamakage, Akiyoshi Namiki
Jun 11, 2009·British Journal of Anaesthesia·H C Hemmings
Nov 12, 2014·The Journal of General Physiology·Karl F HeroldHugh C Hemmings
Mar 2, 1999·The Journal of Physiology·D H Triyoso, T A Good
Jul 28, 2015·Autonomic Neuroscience : Basic & Clinical·S L JohansenM B Harris
Sep 28, 2004·Anesthesiology·Alexandra CorralesEsperanza Recio-Pinto
Nov 25, 2005·Anesthesiology·Kevin J GingrichThomas J Blanck
Jul 29, 2003·Anesthesiology·Igor M NikonorovEsperanza Recio-Pinto

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