Abstract
1. The effects of unfractionated heparin (UH) and a selectively O-desulphated derivative of heparin (ODSH), lacking anticoagulant activity, on the adhesion of human peripheral blood mononuclear cells (HPBMNC) to human stimulated umbilical vein endothelial cells (HUVECs), were investigated. 2. For comparison, the effects of poly-L-glutamic acid (PGA), a large polyanionic molecule without sulphate groups and two different molecular weight sulphated dextrans (DS 5 k and DS 10 k) were studied. 3. UH (50 - 1000 u ml(-1)) significantly (P<0.05) inhibited the adhesion of HPBMNC to HUVECs, stimulated with IL-1beta (100 u ml(-1)), TNF-alpha (1000 u ml(-1)) or LPS (100 microg ml(-1)), when the drugs were added together with stimuli to HUVECs and coincubated for 6 h. Such effects on adhesion occurred with limited influence on expression of relevant endothelial adhesion molecules (ICAM-1 and VCAM-1). 4. UH (100 - 1000 u ml(-1)), when added to prestimulated HUVECs, significantly (P<0.05) increased adhesion of mononuclear cells to endothelium at the higher concentrations tested, without any effect on adhesion molecule expression. In contrast, the opposite effect was observed when human polymorphonuclear leucocyte adhesion was examined, under...Continue Reading
References
Sep 1, 1976·Clinical Immunology and Immunopathology·R Strunk, H R Colten
Feb 1, 1976·Thrombosis Research·L M Hiebert, L B Jaques
Sep 1, 1991·Clinical Science·R L WalshG McLennan
Jan 1, 1991·Allergy·K FredensP Venge
Mar 1, 1990·European Journal of Immunology·O LiderY Naparstek
Sep 15, 1986·The Biochemical Journal·T BârzuJ P Caen
Mar 1, 1989·The Journal of Clinical Investigation·O LiderI R Cohen
Dec 22, 1989·Cell·L OsbornR Lobb
Jan 1, 1988·Annals of the New York Academy of Sciences·D O Willenborg, C R Parish
Jun 1, 1988·The Biochemical Journal·F RediniW Hornebeck
Dec 7, 1973·Nature·P S DamusR D Rosenberg
Jan 1, 1980·International Journal of Immunopharmacology·A BellaviaH S Micklem
Nov 1, 1983·Cellular Immunology·M S SyB Benacerraf
Sep 1, 1995·The Journal of Cell Biology·M S DiamondT A Springer
Sep 1, 1993·British Journal of Pharmacology·M SasakiC P Page
Jan 1, 1994·Seminars in Thrombosis and Hemostasis·L SilvestroR Fantozzi
Dec 1, 1993·British Journal of Pharmacology·M M Teixeira, P G Hellewell
Jul 5, 1996·The Journal of Biological Chemistry·B M RevelleP J Beck
Feb 24, 1997·The Journal of Cell Biology·L GiuffrèO Spertini
Jun 1, 1997·American Journal of Respiratory and Critical Care Medicine·T AhmedC M Svahn
Feb 12, 1998·British Journal of Pharmacology·X XieL Lindbom
Mar 21, 1998·The Journal of Clinical Investigation·A KoenigA Varki
Feb 4, 1999·Journal of Applied Physiology·C CampoT Ahmed
May 20, 1999·Advances in Pharmacology·D J TyrrellC P Page
Nov 24, 1999·Atherosclerosis·A SchmidtE Buddecke
Mar 11, 2000·British Journal of Pharmacology·R LeverC P Page
May 8, 2000·Journal of Applied Physiology·T AhmedC Campo
Jun 22, 2000·Gut·A SalasJ Panés
Mar 29, 2001·Pulmonary Pharmacology & Therapeutics·E A Seeds, C P Page
Citations
Jun 21, 2002·Biochemical and Biophysical Research Communications·Kevin J WoollardHelen R Griffiths
Jun 15, 2005·Annual Review of Biochemistry·T M HandelA E Proudfoot
Jan 2, 2010·European Journal of Pharmacology·Rebecca LeverClive P Page
Mar 4, 2006·British Journal of Haematology·Hélène HochartBarry White
May 30, 2007·British Journal of Pharmacology·R LeverC P Page
Jun 12, 2010·Journal of Biomedical Materials Research. Part a·T S Ramyaa LakshmiMary Babu
Aug 29, 2013·ISRN Pharmacology·Clive Page
May 26, 2016·Pulmonary Pharmacology & Therapeutics·Derek ThomsonClive P Page
Dec 11, 2014·Critical Care Medicine·Ryan ZarychanskiUNKNOWN Canadian Critical Care Trials Group
Jun 8, 2018·Journal of Thrombosis and Haemostasis : JTH·J M WatJ C Kingdom
Jul 18, 2002·Nature Reviews. Drug Discovery·Rebecca Lever, Clive P Page
Jul 18, 2018·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Xu LiXiaochun Ma
May 8, 2007·Shock·Chiu-Li YehSung-Ling Yeh
Mar 23, 2006·Shock·Chiu-Li YehSung-Ling Yeh
Jan 30, 2007·Pharmacology·Ileana ManduteanuMaya Simionescu
Apr 18, 2014·American Journal of Physiology. Cell Physiology·Rebecca LeverClive P Page
Sep 19, 2019·Biomedical Materials·Chaojing LiLu Wang
May 22, 2019·Frontiers in Oncology·Alyce J MayfoshMark D Hulett
Dec 17, 2015·Pharmacological Reviews·Barbara MulloyClive P Page