The effects of heparin on the adhesion of human peripheral blood mononuclear cells to human stimulated umbilical vein endothelial cells

British Journal of Pharmacology
Amir SmailbegovicClive P Page

Abstract

1. The effects of unfractionated heparin (UH) and a selectively O-desulphated derivative of heparin (ODSH), lacking anticoagulant activity, on the adhesion of human peripheral blood mononuclear cells (HPBMNC) to human stimulated umbilical vein endothelial cells (HUVECs), were investigated. 2. For comparison, the effects of poly-L-glutamic acid (PGA), a large polyanionic molecule without sulphate groups and two different molecular weight sulphated dextrans (DS 5 k and DS 10 k) were studied. 3. UH (50 - 1000 u ml(-1)) significantly (P<0.05) inhibited the adhesion of HPBMNC to HUVECs, stimulated with IL-1beta (100 u ml(-1)), TNF-alpha (1000 u ml(-1)) or LPS (100 microg ml(-1)), when the drugs were added together with stimuli to HUVECs and coincubated for 6 h. Such effects on adhesion occurred with limited influence on expression of relevant endothelial adhesion molecules (ICAM-1 and VCAM-1). 4. UH (100 - 1000 u ml(-1)), when added to prestimulated HUVECs, significantly (P<0.05) increased adhesion of mononuclear cells to endothelium at the higher concentrations tested, without any effect on adhesion molecule expression. In contrast, the opposite effect was observed when human polymorphonuclear leucocyte adhesion was examined, under...Continue Reading

References

Sep 1, 1976·Clinical Immunology and Immunopathology·R Strunk, H R Colten
Feb 1, 1976·Thrombosis Research·L M Hiebert, L B Jaques
Sep 1, 1991·Clinical Science·R L WalshG McLennan
Jan 1, 1988·Annals of the New York Academy of Sciences·D O Willenborg, C R Parish
Jun 1, 1988·The Biochemical Journal·F RediniW Hornebeck
Dec 7, 1973·Nature·P S DamusR D Rosenberg
Sep 1, 1995·The Journal of Cell Biology·M S DiamondT A Springer
Jan 1, 1994·Seminars in Thrombosis and Hemostasis·L SilvestroR Fantozzi
May 20, 1999·Advances in Pharmacology·D J TyrrellC P Page

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Citations

Jun 21, 2002·Biochemical and Biophysical Research Communications·Kevin J WoollardHelen R Griffiths
Jun 15, 2005·Annual Review of Biochemistry·T M HandelA E Proudfoot
May 30, 2007·British Journal of Pharmacology·R LeverC P Page
Jun 12, 2010·Journal of Biomedical Materials Research. Part a·T S Ramyaa LakshmiMary Babu
Aug 29, 2013·ISRN Pharmacology·Clive Page
Dec 11, 2014·Critical Care Medicine·Ryan ZarychanskiUNKNOWN Canadian Critical Care Trials Group
Jun 8, 2018·Journal of Thrombosis and Haemostasis : JTH·J M WatJ C Kingdom
Jul 18, 2002·Nature Reviews. Drug Discovery·Rebecca Lever, Clive P Page
Jul 18, 2018·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Xu LiXiaochun Ma
Apr 18, 2014·American Journal of Physiology. Cell Physiology·Rebecca LeverClive P Page
May 22, 2019·Frontiers in Oncology·Alyce J MayfoshMark D Hulett
Dec 17, 2015·Pharmacological Reviews·Barbara MulloyClive P Page

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