The Effects of Herbicides Targeting Aromatic and Branched Chain Amino Acid Biosynthesis Support the Presence of Functional Pathways in Broomrape

Frontiers in Plant Science
Evgenia DorJoseph Hershenhorn

Abstract

It is not clear why herbicides targeting aromatic and branched-chain amino acid biosynthesis successfully control broomrapes-obligate parasitic plants that obtain all of their nutritional requirements, including amino acids, from the host. Our objective was to reveal the mode of action of imazapic and glyphosate in controlling the broomrape Phelipanche aegyptiaca and clarify if this obligatory parasite has its own machinery for the amino acids biosynthesis. P. aegyptiaca callus was studied to exclude the indirect influence of the herbicides on the parasite through the host plant. Using HRT - tomato plants resistant to imidazolinone herbicides, it was shown that imazapic is translocated from the foliage of treated plants to broomrape attachments on its roots and controls the parasite. Both herbicides inhibited P. aegyptiaca callus growth and altered the free amino acid content. Blasting of Arabidopsis thaliana 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) and acetolactate synthase (ALS) cDNA against the genomic DNA of P. aegyptiaca yielded a single copy of each homolog in the latter, with about 78 and 75% similarity, respectively, to A. thaliana counterparts at the protein level. We also show for the first time that both E...Continue Reading

References

Jan 1, 1990·Critical Reviews in Biochemistry and Molecular Biology·R Bentley
Mar 1, 1984·The Journal of Antimicrobial Chemotherapy·W W Nichols
Jul 18, 2000·Journal of Agricultural and Food Chemistry·L J VeldhuisJ C Hall
Feb 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·E SchönbrunnW Kabsch
Feb 28, 2002·The Journal of Infectious Diseases·Craig W RobertsRima McLeod
Mar 3, 2004·Journal of Experimental Botany·W J ZhouD M Joel
Feb 1, 2008·Plant Physiology and Biochemistry : PPB·Ronald G DugglebyLuke W Guddat
Nov 28, 2008·Plant Physiology and Biochemistry : PPB·Zouhaier AbbesPhilippe Simier
Dec 27, 2008·Journal of Plant Physiology·Alexander UlanovVera Lozovaya
Jul 16, 2010·Current Opinion in Plant Biology·John I Yoder, Julie D Scholes
Nov 10, 2011·Plant Methods·Mónica Fernández-AparicioJames H Westwood
Jul 22, 2016·Planta·Tal ShiloHanan Eizenberg

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Methods Mentioned

BETA
transgenic
Assay

Software Mentioned

Agilent GC / MSD Productivity ChemStation
Plot
SPSS
Muscle
DNAMAN
ChloroP
Sigma
JMP
Masshunter

Related Concepts

Related Feeds

Amyloid Lateral Sclerosis

Amyotrophic Lateral Sclerosis (ALS) is a progressive nervous system disease associated with the death of neurons that control voluntary muscles. Discover the latest research on ALS here.