The effects of human amniotic fluid and different bone grafts on vertebral fusion in an experimental rat model

Current Therapeutic Research, Clinical and Experimental
Mithat OnerSevki Erdem

Abstract

The high risk of nonunion represents a challenge in vertebral surgery, thus stimulating new strategies to improve fusion rates. We investigated the effect of 2 different bone grafts and amniotic fluid application on radiologically and histologically evaluated vertebral fusion in an experimental rat model. Forty-eight 24-week-old Sprague Dawley rats were included and assigned into 1 of 4 groups: allograft group, allograft plus human amniotic fluid group, demineralized bone matrix (DBM) group, or DBM plus human amniotic fluid group. After decortication and L4-L6 spinal fusion, study treatments were applied. Fusion in each rat was examined radiologically and histologically 8 weeks after the intervention. The group that received only allograft had better radiologic scores (median = 3.5; range = 3-4) when compared with the group that received only DBM (median = 2; range = 1-4) (P = 0.002); however, histologic scores did not differ. When amniotic fluid was added to the grafting, allograft-based treatments performed better than DBM-based treatments both on radiologic (median = 4; range = 3-4 vs median = 3; range = 3-4; P = 0.003) and histologic (median = 7; range = 6-7 vs median = 5; range = 3-6; P < 0.001) evaluation. Addition of amn...Continue Reading

References

Sep 1, 1976·Clinical Orthopaedics and Related Research·M D BrownP B Davis
Dec 1, 1992·Journal of Spinal Disorders·L G LenkeP L Schoenecker
Oct 1, 1984·The Journal of Clinical Endocrinology and Metabolism·T J MerimeeJ E Tyson
Dec 15, 1995·Spine·V K Sonntag, F F Marciano
Apr 4, 2001·The Journal of Bone and Joint Surgery. British Volume·D HannoucheL Sedel
Jun 12, 2004·British Journal of Plastic Surgery·Güzin Yeşim OzgenelMesut Ozcan
May 27, 2005·The Journal of Surgical Research·Naci KaraçalNecmettin Kutlu
Oct 9, 2012·The Surgeon : Journal of the Royal Colleges of Surgeons of Edinburgh and Ireland·B AghdasiJ C Wang
Mar 22, 2014·World Journal of Orthopedics·Konstantinos TilkeridisGeorgios I Drosos

❮ Previous
Next ❯

Citations

Sep 19, 2019·Acta orthopaedica et traumatologica turcica·Enes SarıHanife Özkayalar

❮ Previous
Next ❯

Software Mentioned

SPSS

Related Concepts

Related Feeds

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.