The effects of hyaluronate-containing medium on human embryo attachment to endometrial epithelial cells in vitro.

Human Reproduction Open
Peter T RuaneDaniel R Brison

Abstract

Does embryo transfer medium containing hyaluronate (HA) promote the attachment phase of human embryo implantation? HA-containing medium does not promote human blastocyst attachment to endometrial epithelial cells in vitro. Embryo transfer media containing high concentrations of HA are being used to increase implantation and live birth rates in IVF treatment, although the mechanism of action is unknown. Expression of HA-interacting genes in frozen-thawed oocytes/embryos was assessed by microarray analysis (n = 21). Fresh and frozen human blastocysts (n = 98) were co-cultured with human endometrial epithelial Ishikawa cell layers. Blastocyst attachment and the effects of a widely used HA-containing medium were measured. Human embryos surplus to treatment requirements were donated with informed consent from several ART centres. Blastocyst-stage embryos were transferred at day 6 to confluent Ishikawa cell layers; some blastocysts were artificially hatched. Blastocyst attachment was monitored from 1 to 48 h, and the effects of blastocyst pre-treatment for 10 min with HA-containing medium were determined. Human embryos expressed the HA receptor genes CD44 and HMMR, hyaluronan synthase genes HAS1-3, and hyaluronidase genes HYAL1-3, at...Continue Reading

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Citations

Mar 23, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Chow-Seng KongPaul J Brighton

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Methods Mentioned

BETA
PCR

Software Mentioned

SPSS
Mas5

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