The effects of hyaluronic acid incorporated as a wetting agent on lysozyme denaturation in model contact lens materials

Journal of Biomaterials Applications
Andrea WeeksHeather Sheardown

Abstract

Conventional and silicone hydrogels as models for contact lenses were prepared to determine the effect of the presence of hyaluronic acid on lysozyme sorption and denaturation. Hyaluronic acid was loaded into poly(2-hydroxyethyl methacrylate) and poly(2-hydroxyethyl methacrylate)/TRIS--methacryloxypropyltris (trimethylsiloxy silane) hydrogels, which served as models for conventional and silicone hydrogel contact lens materials. The hyaluronic acid was cross-linked using 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide in the presence of dendrimers. Active lysozyme was quantified using a Micrococcus lysodeikticus assay while total lysozyme was determined using 125-I radiolabeled protein. To examine the location of hyaluronic acid in the gels, 6-aminofluorescein labeled hyaluronic acid was incorporated into the gels using 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide chemistry and the gels were examined using confocal laser scanning microscopy. Hyaluronic acid incorporation significantly reduced lysozyme sorption in poly(2-hydroxyethyl methacrylate) (p < 0.00001) and poly(2-hydroxyethyl methacrylate)/TRIS--methacryloxypropyltris (trimethylsiloxy silane) (p < 0.001) hydrogels, with the modified materials sorbing only 20% and 16% th...Continue Reading

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Citations

Sep 17, 2013·Biomaterials·Moira C VynerBrian G Amsden
Sep 4, 2013·Contact Lens & Anterior Eye : the Journal of the British Contact Lens Association·Charles J WhiteMark E Byrne
Jan 18, 2015·Journal of Colloid and Interface Science·Lokendrakumar C BenganiAnuj Chauhan
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Dec 17, 2014·Journal of Biomedical Materials Research. Part a·Stefan M PatersonHeather Sheardown
Jun 21, 2016·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Hong ZhaoJun Wu
Apr 19, 2015·Journal of Biomaterials Applications·Myrto KorogiannakiHeather Sheardown
Nov 3, 2020·Journal of Biomaterials Applications·Petar Borisov EftimovGeorgi Asenov Georgiev
Jun 9, 2020·Contact Lens & Anterior Eye : the Journal of the British Contact Lens Association·Katsuhide YamasakiLyndon Jones
Mar 7, 2021·Carbohydrate Polymers·Gloria Huerta Ángeles, Kristina Nešporová
May 1, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Wan-Hsin ChangYuan-Hao Howard Hsu
Dec 13, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Myrto KorogiannakiHeather Sheardown

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Methods Mentioned

BETA
ELISA
Fluorescence

Software Mentioned

ImageJ
Zeiss custom

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