The Effects of Hyperacute Serum on the Elements of the Human Subchondral Bone Marrow Niche

Stem Cells International
Melinda SimonZsombor Lacza

Abstract

Mesenchymal stem cells (MSCs) are widely used in laboratory experiments as well as in human cell therapy. Their culture requires animal sera like fetal calf serum (FCS) as essential supplementation; however, animal sera pose a risk for clinical applications. Human blood derivatives, for example, platelet-rich plasma (PRP) releasates, are potential replacements of FCS; however, it is unclear which serum variant has the best effect on the given cell or tissue type. Additionally, blood derivatives are commonly used in musculoskeletal diseases like osteoarthritis (OA) or osteonecrosis as "proliferative agents" for the topical MSC pool. Hyperacute serum (HAS), a new serum derivative, has been designed to approximate the natural coagulation cascade with a single-step, additive-free preparation method. We investigated the effects of HAS on monolayer MSC cultures and in their natural niche, in 3D subchondral bone and marrow explants. Viability measurements, RT-qPCR evaluation for gene expression and flow cytometry for cell surface marker analysis were performed to compare the effects of FCS-, PRP-, or HAS-supplemented culture media. Monolayer MSCs showed significantly higher metabolic activity following 5 days' incubation in HAS, and o...Continue Reading

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Citations

Jun 11, 2020·International Journal of Molecular Sciences·Svetlana MiroshnichenkoAnastasiya Solovieva
Aug 29, 2020·Tissue Engineering. Part a·Adél HinsenkampIstván Hornyák
Feb 13, 2019·International Journal of Molecular Sciences·Dorottya KardosIstván Hornyák
Jul 25, 2021·International Journal of Molecular Sciences·Isabel Olmos CalvoZsombor Lacza
Jul 22, 2021·Current Issues in Molecular Biology·Olga Kuten-PellaZsombor Lacza
Jul 22, 2021·Current Issues in Molecular Biology·Isabel Olmos CalvoZsombor Lacza
Nov 25, 2021·Infectious Diseases and Therapy·Eszter FodorZsombor Lacza

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Methods Mentioned

BETA
blood collection
Profiler
chips
biopsies
FCS
flow cytometry
confocal microscopy
fluorescence microscopy

Software Mentioned

ImageJ

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