The Effects of Indian Hedgehog Deletion on Mesenchyme Cells: Inducing Intermediate Cartilage Scaffold Ossification to Cause Growth Plate and Phalange Joint Absence, Short Limb, and Dwarfish Phenotypes

Stem Cells and Development
Jian SunLei Wei

Abstract

The endochondral ossification plays a critical role in vertebrate limb development and skeletal homeostasis, where limb mesenchyme cells form an intermediate cartilage scaffold that develops into growth plates and then replaced by bone. Although Indian hedgehog (Ihh) is known to control the hypertrophic differentiation process of chondrocytes, its role from the mesenchyme cells to the early stages of chondrogenesis is unclear. To define the function of Ihh in the mesenchymal cell's early stages of chondrogenesis, we specifically delete Ihh in Prx1-expressed mesenchyme cells at E9.5 using Prx1-Cre;Ihhfl/fl;Rosa26-ZsGreen1 mice. We found that deleting Ihh in the mesenchyme cells results in an early and quick ossification of the intermediate cartilage scaffold, causing the growth plate and phalange joint absence, short limbs, and dwarfishness. The green fluorescent protein (GFP)-positive cells derived from deleted Ihh mesenchyme cells overlap with von Kossa- and osteocalcin-positive staining area. These deleted Ihh/GFP-positive cells isolated from Prx1-Cre;Ihhfl/fl;Rosa26-ZsGreen1 newborn mice had osteogenic differentiation by showing a positive Alizarin red and von Kossa staining, as well as an enhanced Col1a1, osteocalcin, and R...Continue Reading

References

Feb 13, 2001·The Journal of Clinical Investigation·U I ChungH M Kronenberg
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jul 12, 2002·Genesis : the Journal of Genetics and Development·Malcolm LoganClifford J Tabin
May 16, 2003·Nature·Henry M Kronenberg
Sep 28, 2005·Journal of Cellular Biochemistry·Lick Pui Lai, Jane Mitchell
Jan 11, 2007·Genesis : the Journal of Genetics and Development·Mo ChenDi Chen
Apr 6, 2007·Proceedings of the National Academy of Sciences of the United States of America·Yukiko MaedaBeate Lanske
Jul 31, 2007·The International Journal of Biochemistry & Cell Biology·E J MackieM Mirams
Aug 21, 2008·Nature Protocols·Marjolaine GossetClaire Jacques
Jun 10, 2010·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Mary L BouxseinRalph Müller
Dec 15, 2011·The Journal of Molecular Diagnostics : JMD·Fay WangYuling Luo
Jan 4, 2013·Cold Spring Harbor Perspectives in Biology·Fanxin Long, David M Ornitz
Aug 10, 2013·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·R S MaJ F Hou
Sep 27, 2013·Folia morphologica·S MoskalewskiA Osiecka-Iwan
Jan 9, 2014·Annals of Clinical Biochemistry·Thinn Thinn Hlaing, Juliet E Compston
Aug 6, 2014·Proceedings of the National Academy of Sciences of the United States of America·Liu YangKathryn S E Cheah
May 30, 2015·International Journal of Oral Science·Jing YangYing-Zi Yang
Jun 23, 2015·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Katsuhiko AmanoBeate Lanske
Sep 6, 2015·Journal of Dental Research·Y JingJ Q Feng

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Citations

Sep 23, 2021·Proceedings of the National Academy of Sciences of the United States of America·Maike GetwanSoeren S Lienkamp

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Methods Mentioned

BETA
targeted mutation
X-ray
flow cytometry
PCR
genotyping
fluorescence-activated cell sorting

Software Mentioned

IQ50

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