PMID: 6411477Jul 1, 1983Paper

The effects of ionizing irradiation on the sedimentation coefficient of cytoplasmic steroid receptors in rat mammary tumors

European Journal of Cancer & Clinical Oncology
J P JanssensW De Loecker

Abstract

The effects of ionizing irradiation on the sedimentation coefficients of both estrogen receptor (ER) and progesterone receptor (PgR) have been examined in comparison to the effects of proteolysis. DMBA-induced rat mammary tumors were subjected to a treatment of 20 Gy and the ER and PgR concentrations were determined at different time intervals after irradiation. On a 5-20% sucrose gradient the ER sedimented as 9-11 and 4-5 S molecular forms, while PgR sedimented as a small 8-9 S peak and a major 4-5 S peak. Radiotherapy particularly reduced the 4-5 S sedimentation peaks of both receptors but did not initiate any new sedimentation forms. Although the 4-5 S ER receptor concentrations remained low, both progesterone receptor forms appeared to recover by 60 days after treatment. As these effects could be due to the release of proteolytic enzymes following irradiation of tumors, the receptors from untreated tumors were exposed to different concentrations of trypsin. The effects of trypsin were identical for ER and for PgR, and proved to be dependent on the trypsin concentration. Only concentrations of trypsin up to 30 micrograms/ml resulted in a reduction of 9-11 S ER or 8-9 S PgR forms which was accompanied by a simultaneous increa...Continue Reading

References

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Jun 1, 1981·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·J P JanssensW De Loecker
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Citations

Feb 15, 1985·International Journal of Cancer. Journal International Du Cancer·L Van HoveM Vandeputte
Jun 15, 1984·International Journal of Cancer. Journal International Du Cancer·L Van HoveM Vandeputte

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