The effects of kisspeptin-10 on migration and proliferation of endothelial cell

Advanced Biomedical Research
Fatemeh Golzar, Shaghayegh Haghjooy Javanmard

Abstract

Migration, expansion and survival of endothelial cells that are an important cellular component of blood vessels plays an important role in the induction of tumor growth. Kisspeptins (kp), peptides that bind to coupled-G protein receptor (GPR54), inhibit each step of metastatic cascade include invasion, migration and homing, angiogenesis, survival and proliferation. In this study we investigated effects of kisspeptin-10, the most potent member of kisspeptin family, on Migration and proliferation of endothelial cells that are necessary for angiogenesis and tumor metastasis. We compared migration of Human Umbilical Vein Endothelial Cells (HUVECs) were treated with 10-100 or 500 nM kp-10 for 24 hours and no treated cells using an in vitro trans membrane migration assay and HUVEC proliferation of treated endothelial cells with 10-100 or 500 nM kp-10 for 48 hours and no treated cells was measured by MTT Cell Proliferation Assay Kit. Analysis of data was performed using the Kruskal-Wallis test followed by the Mann-Whitney test. Migration and proliferation of endothelial cells were increased at lower concentration of kp-10 specially at 100 nM while higher concentration reduced both migration and proliferation. Our data showed that dif...Continue Reading

Citations

Jan 24, 2017·Laboratory Investigation; a Journal of Technical Methods and Pathology·Jelena LoeglUrsula Hiden
Oct 26, 2016·American Journal of Respiratory and Critical Care Medicine·Silin SaMarlene Rabinovitch
Jul 6, 2017·Reproduction : the Official Journal of the Society for the Study of Fertility·Junyu ZhaiWeiping Li

❮ Previous
Next ❯

Methods Mentioned

BETA
flow cytometry
Assay
transfection

Software Mentioned

SPSS

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Cell Migration in Cancer and Metastasis

Migration of cancer cells into surrounding tissue and the vasculature is an initial step in tumor metastasis. Discover the latest research on cell migration in cancer and metastasis here.