PMID: 2106362Feb 5, 1990Paper

The effects of L-dopa on in vitro dopamine release from striatum

Brain Research
G L Snyder, M J Zigmond

Abstract

We have examined the effects of L-dihydroxyphenylalanine (L-DOPA) on endogenous dopamine (DA) and dihydroxyphenylacetic acid (DOPAC) efflux from superfused striatal slices prepared from adult male rats. Superfusion with L-DOPA (10 microM) caused a modest elevation in the tissue levels of DA and greatly increased the basal efflux and stimulation-evoked overflow of DA. Stimulation of slices under Ca2(+)-free conditions abolished DA overflow occurring in the absence of L-DOPA, but reduced DA overflow in the presence of L-DOPA by only 56%. Ca2(+)-independent DA release was not reduced by nomifensine. Destruction of DA terminals by pretreatment with 6-hydroxydopamine did not alter the capacity of L-DOPA to elevate tissue DA content. However, it attenuated the impact of L-DOPA on DA efflux, although this effect was somewhat smaller than was the apparent loss of DA terminals. These results suggest the following conclusions: (1) L-DOPA increases both the spontaneous and depolarization-induced release of DA; (2) some of the DA formed from L-DOPA can be released in response to depolarization by a process that does not involve either Ca2(+)-dependent exocytosis or reverse transport; and (3) most but not all of the DA efflux occurring in t...Continue Reading

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Citations

Apr 15, 2000·Synapse·S M PearlM J Zigmond
Jan 1, 1993·Journal of Neural Transmission. General Section·J GrossC Berndt
Jul 1, 1993·Journal of Neuroscience Methods·E L GardnerW Paredes
Mar 1, 1992·Neurochemistry International·N B MercuriG Bernardi
Mar 22, 2002·European Journal of Pharmacology·Juan J SanchezM Carmen Gonzalez
Sep 13, 1994·Proceedings of the National Academy of Sciences of the United States of America·R Y ShenL A Chiodo
May 1, 2009·Behavioural Pharmacology·Yusuke KabukiMitsuhiro Furuse
Nov 1, 1995·British Journal of Pharmacology·W Y Chang, R A Webster
May 11, 1992·Annals of the New York Academy of Sciences·M J ZigmondE D Abercrombie
Jul 9, 2016·Progress in Neurobiology·Philippe De DeurwaerdèreMark J Millan

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