The Effects of Low-Dose Bisphenol A and Bisphenol F on Neural Differentiation of a Fetal Brain-Derived Neural Progenitor Cell Line

Frontiers in Endocrinology
Yuki FujiwaraTakahiko Katoh

Abstract

Environmental chemicals are known to disrupt the endocrine system in humans and to have adverse effects on several organs including the developing brain. Recent studies indicate that exposure to environmental chemicals during gestation can interfere with neuronal differentiation, subsequently affecting normal brain development in newborns. Xenoestrogen, bisphenol A (BPA), which is widely used in plastic products, is one such chemical. Adverse effects of exposure to BPA during pre- and postnatal periods include the disruption of brain function. However, the effect of BPA on neural differentiation remains unclear. In this study, we explored the effects of BPA or bisphenol F (BPF), an alternative compound for BPA, on neural differentiation using ReNcell, a human fetus-derived neural progenitor cell line. Maintenance in growth factor-free medium initiated the differentiation of ReNcell to neuronal cells including neurons, astrocytes, and oligodendrocytes. We exposed the cells to BPA or BPF for 3 days from the period of initiation and performed real-time PCR for neural markers such as β III-tubulin and glial fibrillary acidic protein (GFAP), and Olig2. The β III-tubulin mRNA level decreased in response to BPA, but not BPF, exposure....Continue Reading

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Citations

Aug 5, 2020·Journal of Neuroendocrinology·Pascal CoumailleauThierry D Charlier
Mar 26, 2021·Frontiers in Cellular Neuroscience·Felipe A Bustamante-BarrientosLuis Federico Bátiz
Apr 25, 2021·European Journal of Pediatrics·Sarah ZulkifliNoor Shafina Mohd Nor

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Methods Mentioned

BETA
PCR

Software Mentioned

GraphPad Prism

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