The effects of lycopene on the proliferation of human breast cells and BRCA1 and BRCA2 gene expression

European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)
Nasséra ChalabiDominique Bernard-Gallon

Abstract

The purpose of this study was to demonstrate the effects of lycopene, the major tomato carotenoid, on the expression of the BRCA1 and BRCA2 genes in three breast tumour cell lines, MCF-7, HBL-100, MDA-MB-231 and the fibrocystic breast cell line MCF-10a. Flow cytometry analysis showed a G(1)/S phase cell cycle-arrest after treatment of the cells with 10 microM lycopene for 48 h. mRNA expression was studied by quantitative reverse transcription-polymerase chain reaction using the Taqman method. We observed an increase of BRCA1 and BRCA2 mRNA in the oestrogen receptor (ER)-positive cell lines (MCF-7 and HBL-100), and a decrease (MDA-MB-231) or no change (MCF-10a) in the ER-negative cell lines. BRCA1 and BRCA2 proteins were quantified by perfusion affinity chromatography. No variation in their expression was observed. These preliminary results on the effects of lycopene on the expression of BRCA1 and BRCA2 oncosuppressor genes in breast cancer may reflect cross-talk between the oestrogen and retinoic acid receptor (RAR) pathways.

References

Jan 1, 1997·Molecular and Cellular Biology·S ThakurB L Weber
Apr 1, 1997·Journal of the American College of Nutrition·H Gerster
Nov 11, 1998·Nature Medicine·L FinkR M Bohle
Mar 3, 1999·Journal of Chromatography. B, Biomedical Sciences and Applications·C HizelD J Bernard-Gallon
Sep 28, 1999·Oncogene·I BiècheR Lidereau
Mar 18, 2000·Toxicology Letters·M M MansonS A Prigent
Jul 25, 2000·Biochemical and Biophysical Research Communications·D A FavyD Bernard-Gallon
Aug 5, 2000·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·C X Deng, S G Brodie
Oct 7, 2000·Journal of the American College of Nutrition·A V Rao, S Agarwal
Jan 9, 2001·International Journal of Cancer. Journal International Du Cancer·F LeviC La Vecchia
Feb 15, 2001·JAMA : the Journal of the American Medical Association·S A Smith-WarnerD J Hunter
May 12, 2001·The Breast Journal·Michael S. SimonLance K. Heilbrun
Jun 21, 2001·American Journal of Epidemiology·P TonioloE Riboli
Jan 28, 2003·Cancer·Judith Clancy Keen, Nancy E Davidson
May 10, 2003·Journal of Women's Health·George L BlackburnRita B Buckley
Oct 31, 2003·Molecular Aspects of Medicine·Volker BöhmRoland Bitsch

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Citations

Jun 18, 2009·European Journal of Nutrition·Anderson J TeodoroRadovan Borojevic
Mar 10, 2010·The British Journal of Nutrition·Myriam RichelleGary Williamson
Dec 25, 2007·Antioxidants & Redox Signaling·Naghma KhanHasan Mukhtar
Mar 12, 2008·Carcinogenesis·Andreas WoelfelschneiderPeter Schmezer
Jan 29, 2008·American Journal of Therapeutics·Soley SerenOmer Kucuk
Aug 5, 2006·Pharmacogenomics·Nasséra ChalabiDominique Bernard-Gallon
Jul 3, 2013·Nutrition Reviews·Pushpinder KaurDavid E Williams
Sep 4, 2008·Pediatric Surgery International·Henry L ChangAllan M Goldstein
Jan 31, 2015·International Journal of Molecular Sciences·Atsuko NakanishiSatoru Matsuda
Jun 16, 2015·Environment International·Ryoiti Kiyama, Yuko Wada-Kiyama
Dec 5, 2006·Toxicology in Vitro : an International Journal Published in Association with BIBRA·F FornelliG Zacheo
Oct 26, 2007·Biochemical and Biophysical Research Communications·Delphine GitenayEdmond Rock
May 6, 2014·Frontiers in Plant Science·Paolo PesaresiSimona Masiero
Feb 7, 2014·Critical Reviews in Food Science and Nutrition·M Viuda-MartosJ Fernández-López
Jun 15, 2010·The Breast : Official Journal of the European Society of Mastology·Jessica DennisUNKNOWN Hereditary Breast Cancer Clinical Study Group
Oct 30, 2016·British Journal of Pharmacology·Alireza MilaniAzam Bolhassani
Apr 18, 2007·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Nasséra ChalabiDominique J Bernard-Gallon
Jan 1, 2011·Cancers·Paola PalozzaMaria Cristina Mele
Jan 1, 2008·Personalized Medicine·Nasséra ChalabiYves-Jean Bignon

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