The effects of mechanical stimulation on the distribution of beta 1 integrin and expression of beta 1-integrin mRNA in TE-85 human osteosarcoma cells

Archives of Oral Biology
R S CarvalhoE H Yen

Abstract

Mechanical stimulation of the skeleton alters the metabolism of bone cells, but the effects of mechanical strain on the cytoskeleton of osteoblasts are poorly understood. While changes in the distribution of the cytoskeleton in mechanically strained cells have been reported, little is known about the pathways by which these changes are transduced into cell functions. Human osteosarcoma (HOS) TE-85 cells were cultured in Dubelcco's modified Eagle's medium/F-12 and grown to confluency in Flexercell type I dishes in a humidified incubator with 5% CO2 and 95% air. Intermittent strain (3 cycles/min) was applied to the cells for periods of 15 and 30 min, 2, 4 and 24 h, and 3, 5, 7, 10, 14, 20 and 28 days. Unstrained cells were used as controls. The distribution of beta 1 integrin was studied immunocytochemically. Total RNA was isolated at every period of time and Northern blots were used to study the effects of strain on the levels of beta 1-integrin expression. The results indicated that mechanical strain increased the synthesis of beta 1 integrin. Northern blots showed that beta 1 mRNA expression was increased significantly (p < 0.005) at 30 min and 3 days of strain application. Strain also affected beta 1 distribution markedly in ...Continue Reading

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