The effects of microwave-irradiated fixation for postmortem changes of the kidney

The Journal of Veterinary Medical Science
Akira YabukiSyusaku Suzuki

Abstract

In the present study, we evaluated the advantages of microwave-irradiated fixation for postmortem autolysis of the kidney. Mouse kidneys, sampled at 0, 1, 3, 5, 10, 15, 20 and 25 hr after death, were fixed with 10% neutral formalin by microwave irradiation (MWI; 20 sec/500 W) and by conventional immersion. They were then examined with light and electron microscopy, morphometrics and immunohistochemicals. Light microscopic and morphometric observations showed that structural preservation effect of MWI was limited to the proximal convoluted tubules at 25 hr. Contrary, mild ultrastructural damage by MWI was found in the glomeruli at 0 and 15 hr. Immunohistochemistry for renin and alpha-smooth muscle actin showed no apparent differences between MWI and the immersion.

References

Aug 1, 1985·The Journal of Pathology·A S LeongJ Milios
Sep 1, 1985·The Journal of Pathology·I E el-ShennawyS R Aparicio
Apr 1, 1970·Journal of Clinical Pathology·C P Mayers
May 2, 2002·Kidney International. Supplement·Marcin AdamczakEberhard Ritz
Aug 16, 2002·Medical Electron Microscopy : Official Journal of the Clinical Electron Microscopy Society of Japan·Mohammed S Razzaque, Takashi Taguchi
Aug 16, 2002·Medical Electron Microscopy : Official Journal of the Clinical Electron Microscopy Society of Japan·Keisuke InaTatsuo Shimada
Dec 6, 2002·Journal of the American Society of Nephrology : JASN·Massimo VolpeSperanza Rubattu
Jun 12, 2004·Photochemistry and Photobiology·P K ChowdhuryJ W Petrich

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