Apr 24, 2020

The hemolymph of Biomphalaria snail vectors of schistosomiasis supports a diverse microbiome

BioRxiv : the Preprint Server for Biology
F. D. ChevalierWinka Le Clec'h

Abstract

The microbiome - the microorganism community that is found on or within an organism's body - is increasingly recognized to shape many aspects of its host biology and is a key determinant of health and disease. Microbiomes modulate the capacity of insect disease vectors (mosquitos, tsetse flies, sandflies) to transmit parasites and disease. We investigate the diversity and abundance of microorganisms within the hemolymph (i.e. blood) of Biomphalaria snails, the intermediate host for Schistosoma mansoni, using Illumina MiSeq sequencing of the bacterial 16S V4 rDNA. We sampled hemolymph from 5 snails from 6 different laboratory populations of B. glabrata and one population of B. alexandrina. We observed 281.34 {+/-} 0.81 amplicon sequence variants (ASVs) per snail. There were significant differences in microbiome composition at the level of individual snails, snail populations and species. Snail microbiomes were dominated by Proteobacteria and Bacteroidetes while water microbiomes from snail tank were dominated by Actinobacteria. We investigated the absolute bacterial load using qPCR: hemolymph samples contained 2,620 {+/-} 321 bacteria per L. We speculate that the microbiome may represent a critical, but unexplored intermediary i...Continue Reading

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Mentioned in this Paper

Patterns
Leukocyte Adhesion Deficiency Type 1
Genome
GCNT2 gene
Dysequilibrium Syndrome
Genomics
African American
Local
Anterior Descending Branch of Left Coronary Artery
Genetic Linkage

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