The Effects of miR-195-5p/MMP14 on Proliferation and Invasion of Cervical Carcinoma Cells Through TNF Signaling Pathway Based on Bioinformatics Analysis of Microarray Profiling

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
Min LiHong-Bo Wang

Abstract

This study is aimed at identification of miR-195-5p/MMP14 expression in cervical cancer (CC) and their roles on cell proliferation and invasion profile of CC cells through TNF signaling pathway in CC. Microarray analysis, gene set enrichment analysis (GSEA) and DAVID were used to analyze differentially expressed miRNAs, mRNAs and signaling pathways. MiR-195-5p and MMP14 expression levels in CC cell were determined by qRT-PCR. Western blot was employed to measure MMP14 and TNF signaling pathway-relating protein level. Luciferase reporter system was used to confirm the targeting relationship between MMP14 and miR-195-5p. Cell proliferation and invasion was respectively deeded by CCK8, transwell. In vivo experiment was carried out to study the impact of MMP14 and miR-195-5p on CC development in mice. The microarray analysis and the results of qRT-PCR determined that miR-195-5p was under-expressed and MMP14 was over-expressed in CC cells. GSEA and DAVID analysis showed that TNF signaling pathway was regulated by miR-195-5p/MMP14 and activated in cervical carcinoma cells. The miR-195-5p and MMP14 have a negative regulation relation. In vivo experiment found that down-regulated MMP14 and up-regulated miR-195-5p suppressed the tumor d...Continue Reading

Citations

Nov 27, 2019·Technology in Cancer Research & Treatment·Xinquan WuKuirong Jiang
Jan 22, 2020·Journal of Cellular Physiology·Xueqiang GuoGuangwen Chen
Dec 18, 2020·Acta Biochimica Et Biophysica Sinica·Lina BuShuanying Yang
Nov 7, 2020·Journal of Reproductive Immunology·Minfei JinHong Zhu
Oct 27, 2020·Cancer Biomarkers : Section a of Disease Markers·Chenglin LiYanyu Wu
Jul 10, 2021·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Natalia MorachoCristina Sánchez-Camacho

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