The effects of mitochondrial energetics inhibitors on the fluorescence of potential-sensitive dyes rhodamine 123 and diS-C3-(5) in lymphocyte suspensions

Journal of Bioenergetics and Biomembranes
E N Mokhova, I A Rozovskaya

Abstract

The effects of uncouplers (FCCP, DNF), oligomycin, and rotenone on the fluorescence of potential-sensitive dyes, rhodamine 123 and diS-C3-(5), in lymphocyte suspensions were compared. The fluorescence of these optical probes gradually increased at higher FCCP concentrations. The dependences of fluorescence intensities and FCCP concentrations were similar for both dyes, and only diS-C3-(5) fluorescence started increasing at lower FCCP concentrations. Rotenone (1 microM) significantly increased rhodamine 123 fluorescence. TMPD-induced and uncoupler-induced diS-C3-(5) fluorescence changes increased 1.5- to 2-fold if the incubation mixture was supplemented with oligomycin (0.1-0.2 microgram/ml). The fluorescence responses of the dyes in the lymphocyte suspension correlate with the effects of mitochondrial energetics inhibitors on delta psi m in isolated mitochondria. The results suggest the possibility of using these dyes for estimating the direction of the delta psi m changes in the lymphocyte suspension.

References

Mar 2, 1978·Nature·J B Miller, D E Koshland
Jul 14, 1977·Biochimica Et Biophysica Acta·G Burchkhardt
Aug 8, 1978·Biochimica Et Biophysica Acta·K W Kinnally, H Tedeschi
Jun 30, 1976·The Journal of Membrane Biology·A Waggoner
Mar 20, 1975·Biochimica Et Biophysica Acta·P C LarisR R Chaffee
Apr 8, 1985·FEBS Letters·S A Novgorodov, L A Yaguzhinsky
Dec 17, 1973·European Journal of Biochemistry·E Padan, H Rottenberg
Jun 28, 1973·Biochimica Et Biophysica Acta·R M BertinaE C Slater
Feb 14, 1984·Biochemical and Biophysical Research Communications·J S Modica-NapolitanoJ R Aprille
Oct 1, 1983·Cell Biochemistry and Function·J Bereiter-HahnJ S Ploem
May 15, 1982·The Biochemical Journal·S M Felber, M D Brand
May 15, 1984·Archives of Biochemistry and Biophysics·A PeñaM Borbolla
Oct 29, 1982·Biochemical and Biophysical Research Communications·H M KorchakG Weissmann

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Respiration

This feed focuses on cellular respiration in bacteria, known as bacterial respiration. Discover the latest research here.