The effects of mycoplasma contamination upon the ability to form bioengineered 3D kidney cysts

PloS One
Teresa M DesRochersDavid L Kaplan

Abstract

Mycoplasma contamination of cell cultures is a pervasive, often undiagnosed and ignored problem in many laboratories that can result in reduced cell proliferation and changes in gene expression. Unless contamination is specifically suspected, it is often undetected in two dimensional (2D) cultures and the resulting effects of mycoplasma contamination are rarely appreciated and can lead to incorrect conclusions. Three dimensional (3D) tissue cultures are increasingly utilized to explore tissue development and phenotype. However, 3D cultures are more complex than 2D cell cultures and require a more controlled cellular environment in order to generate structures necessary to mimic in vivo responses and are often maintained for longer time periods. Changes to the microenvironment are assumed to have a more extreme effect upon the success of 3D tissue cultures than 2D cell cultures, but the effects of mycoplasma have not been studied. To test this hypothesis, we grew 2D cell cultures and 3D tissues from pig kidney epithelial cells (LLC-PK1) that were contaminated with mycoplasma and the same stock of cells after mycoplasma removal. We did not observe an effect of mycoplasma contamination on proliferation in 2D monolayer cell culture...Continue Reading

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Citations

Dec 14, 2017·Critical Reviews in Biotechnology·Gloria PadmaperumaSeetharaman Vaidyanathan
Jan 17, 2020·Scientific Reports·Allison L BrillBarbara E Ehrlich

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Methods Mentioned

BETA
phase contrast microscopy
PCR

Software Mentioned

VisionWorks LS

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