The effects of myokines on osteoclasts and osteoblasts

Biochemical and Biophysical Research Communications
Jin Young LeeBeom-Jun Kim

Abstract

Recently, muscle has received much attention as an endocrine organ regulating other biological targets, including the pancreas, liver, and adipose tissue. Although there is a possibility that muscle-secreting factors biochemically affect bone metabolism in a paracrine manner, the net effects of myokines on the biology of osteoclasts and osteoblasts, particularly on bone mass in vivo, have not yet been thoroughly investigated. Therefore, we performed in vitro as well as animal experiments using conditioned media (CM) collected from C2C12 myoblast and myotube cultures to better understand the interactions between muscle and bone. Compared with non-CM (i.e., control) and myoblast CM, myotube CM markedly inhibited in vitro bone resorption through the suppression of osteoclast differentiation and resorptive activity of individual osteoclasts. Consistently, the expressions of osteoclast differentiation markers, such as tartrate-resistant acid phosphatase (Trap) and calcitonin receptor (Ctr), decreased with myotube CM. Myotube CM significantly stimulated preosteoblast viability and migration and reduced apoptosis, thereby resulting in an increase in calvaria bone formation. Importantly, systemic treatment with myotube CM for 4 weeks i...Continue Reading

Citations

Mar 14, 2020·Biomolecules·Marietta HerrmannFranz Jakob
Dec 11, 2020·NPJ Microgravity·Andrew R KrauseHenry J Donahue
Nov 27, 2020·Frontiers in Cell and Developmental Biology·Jin Young LeeBeom-Jun Kim

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