The effects of natural ligands of hormone receptors and their antagonists on telomerase activity in the androgen sensitive prostatic cancer cell line LNCaP

Biochemical Pharmacology
Jan BouchalErwin von Angerer

Abstract

The effects of the 17beta-estradiol, dihydrotestosterone and hormone antagonists tamoxifen and bicalutamide on telomerase activity and expression of cell cycle related proteins in the androgen-sensitive prostatic cancer cell line LNCaP were studied. The cell line was grown in RPMI supplemented with 2.5% charcoal-stripped FBS for 72 hr. The IC(50) of tamoxifen and bicalutamide and the optimal stimulatory concentrations of 17beta-estradiol and dihydrotestosterone were determined by means of the cell-viability assay, the activity of telomerase was measured by the telomere repeat amplification protocol (TRAP) and the expression of proteins was analysed by the Western blot technique. 17beta-estradiol stimulated cell growth more effectively than dihydrotestosterone whereas hormone antagonists tamoxifen and bicalutamide caused a significant decrease in cell viability. The treatment of cells by a combination of low doses of 17 beta-estradiol and dihydrotestosterone stimulated cells stronger than treatment by a single hormone. Only 17beta-estradiol, in concentration of 10nM, increased strongly the expression of p21(Waf1/Cip1) and increased slightly telomerase activity in the LNCaP cells. 50 microM of bicalutamide down-regulated the leve...Continue Reading

References

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Citations

Mar 15, 2003·Clinica Chimica Acta; International Journal of Clinical Chemistry·Monte S Willis, Frank H Wians
Apr 7, 2010·Ageing Research Reviews·Jun-Ping LiuHe Li
Jul 12, 2005·Molecular and Cellular Endocrinology·Sharyn Bayne, Jun-Ping Liu
Nov 15, 2007·Cytogenetic and Genome Research·O Calcagnile, D Gisselsson
Aug 3, 2010·Molecular and Cellular Endocrinology·Mitra NourbakhshFatemeh Karami-Tehrani

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