Abstract
Angiogenesis is critical for peri-implant bone regeneration and osseointegration. Endothelial cells (ECs) play an important role in angiogenesis during the early stage of bone formation. Nerve growth factor (NGF) is also reported to function as an angiogenic growth factor. The effects of NGF on ECs seeded on titanium surfaces are unclear. This study was done to investigate the influence of NGF on peri-implant angiogenesis in vitro and in vivo. We used two different titanium surfaces. ECs seeded on these surfaces were treated with indicated concentrations of NGF or vascular endothelial growth factor (VEGF). Proliferation, differentiation, morphological features, and amounts attached were assessed. Chicken embryo chorioallantoic membrane (CAM) was adopted to evaluate the effect of NGF in vivo. The results showed that NGF could promote EC proliferation on both titanium surfaces (F1d=2.083, P=0.156; F3d=30.857, P=0.0002; F5d=4.440, P=0.041; F7d=11.065, P=0.001). NGF and the SLA surface upregulated mRNA of NGF, TrkA, and p75 expression (FNGF=11.941, P=0.003; FTrkA=28.514, P=0.004; Fp75=7.725, P=0.01). In vivo, the supernatants of the NGF-treated group could promote neovascularization in CAM (F=17.662, P=0.009). This study demonstrat...Continue Reading
References
Apr 26, 1991·Science·D R KaplanL F Parada
Aug 1, 1990·The Journal of Clinical Investigation·S KourembanasD V Faller
Sep 4, 1987·Science·R Levi-Montalcini
May 1, 1995·Cell Biology International·J L Scully, U Otten
Jul 1, 1994·Journal of Cellular Biochemistry·P Collin-Osdoby
Apr 29, 1998·International Journal of Oncology·R KumarI J Fidler
Nov 10, 1998·Experimental Cell Research·T Goto, D M Brunette
Feb 23, 1999·Clinical and Experimental Pharmacology & Physiology·D V Faller
Mar 22, 2001·Proceedings of the National Academy of Sciences of the United States of America·L CalzaR Levi-Montalcini
Aug 3, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Giuseppina CantarellaRenato Bernardini
Jun 26, 2004·Journal of Dental Research·D BuserS G Steinemann
Jan 19, 2006·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Maria Luisa Brandi, Patricia Collin-Osdoby
Apr 26, 2006·Journal of Biomedical Materials Research. Part a·S J FergusonD Buser
Aug 2, 2007·The Journal of Biological Chemistry·Myung-Jin ParkSeok-Il Hong
Aug 12, 2008·Seminars in Cell & Developmental Biology·Aaron SchindelerDavid G Little
Sep 10, 2008·Angiogenesis·Anca Maria CimpeanMarius Raica
Nov 6, 2008·Journal of Biomedical Materials Research. Part a·K AbshagenB Vollmar
Aug 12, 2009·Clinical Oral Implants Research·Ingemar Abrahamsson, Tord Berglundh
Jun 2, 2012·Zygote : the Biology of Gametes and Early Embryos·R N ChavesJ R Figueiredo
Sep 19, 2012·Bone·Ying-ying WuPing Gong
Mar 22, 2013·Journal of Bone and Mineral Metabolism·Vinícius C SouzaOtávio T Nóbrega
Apr 25, 2013·Cytokine & Growth Factor Reviews·Shek Man ChimJiake Xu
May 7, 2013·Journal of Cardiovascular Pharmacology·Andreas KaratzasNikos E Tsopanoglou
Jun 6, 2013·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Christopher J Percival, Joan T Richtsmeier
Oct 15, 2013·Journal of Biomedical Materials Research. Part a·Xiaojie LiGang Sun
Jul 19, 2014·Archives of Biochemistry and Biophysics·Uttara SaranSuvro Chatterjee