The effects of neuregulin-1β on intrafusal muscle fiber formation in neuromuscular coculture of dorsal root ganglion explants and skeletal muscle cells

Skeletal Muscle
Yuan QiaoZhenzhong Li

Abstract

The formation of intrafusal muscle (IM) fibers and their contact with afferent proprioceptive axons is critical for construction, function, and maintenance of the stretch reflex. Many factors affect the formation of IM fibers. Finding new factors and mechanisms of IM fiber formation is essential for the reconstruction of stretch reflex arc after injury. We established a coculture system of organotypic dorsal root ganglion (DRG) explants and dissociated skeletal muscle (SKM) cells. The formation of IM fibers was observed in this coculture system after neuregulin-1β (NRG-1β) incubation. We found that NRG-1β promoted outgrowth of neurites and migration of neurons from the organotypic DRG explants and that this correlated with an induction of growth-associated protein 43 (GAP-43) expression. NRG-1β also increased the amount of nuclear bag fibers and nuclear chain fibers by elevating the proportion of tyrosine kinase receptor C (TrkC) phenotypic DRG neurons. In addition, we found that the effects of NRG-1β could be blocked by inhibiting ERK1/2, PI3K/Akt, and JAK2/STAT3 signaling pathways. These data imply that NRG-1β promoted neurite outgrowth and neuronal migration from the organotypic DRG explants and that this correlated with an ...Continue Reading

References

Dec 4, 2008·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Lihong WangZhenzhong Li
Dec 23, 2009·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Lisa J KirkpatrickBenjamin W C Rosser
Jul 16, 2010·European Journal of Neurology : the Official Journal of the European Federation of Neurological Societies·R L Rosales, D Dressler
Mar 4, 2011·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Catharina OsterlundPer-Olof Eriksson
May 9, 2012·Current Opinion in Neurobiology·David J Solecki
Feb 5, 2013·The European Journal of Neuroscience·Stefano SartiniRiccardo Cuppini
Jun 25, 2013·The EMBO Journal·Cyril CheretCarmen Birchmeier
Sep 18, 2013·Proceedings of the National Academy of Sciences of the United States of America·Arthur W EnglishKeqiang Ye
Feb 26, 2014·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·M D SannaN Galeotti
May 30, 2014·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Yoon Kyung ShinHwan Tae Park
Jul 30, 2014·Developmental Biology·Yina ZhangStephan Kröger
Aug 2, 2014·Journal of Tissue Engineering and Regenerative Medicine·Víctor CarrielMiguel Alaminos
Nov 5, 2014·Muscle & Nerve·Kevin Peikert, Christian Albrecht May
Nov 8, 2014·The European Journal of Neuroscience·Yina ZhangStephan Kröger
Nov 25, 2014·Nature Neuroscience·Joel W BlanchardKristin K Baldwin
Dec 17, 2014·Cytotechnology·Ruifeng LiDianming Jiang
Jan 27, 2015·Biochemical and Biophysical Research Communications·Elena Zvaritch, David H MacLennan
Apr 10, 2015·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Michelle Oliveira Fernandes, Warren G Tourtellotte
May 23, 2015·Journal of Anatomy·Dina RadovanovicFatima Pedrosa Domellöf
Jun 11, 2015·Developmental Neurobiology·Yuuki OgiharaTakashi Shiga
Jul 17, 2015·Skeletal Muscle·Stefano SchiaffinoCarlo Reggiani
Sep 19, 2015·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Rui HuangQunyuan Xu
Oct 1, 2015·Journal of Cranio-maxillo-facial Surgery : Official Publication of the European Association for Cranio-Maxillo-Facial Surgery·Mehmet CeberMerve Karakas
Mar 13, 2016·Current Topics in Developmental Biology·Carmen Birchmeier, David L H Bennett
Nov 9, 2016·Neuroscience Research·Ken MuramatsuSei-Ichi Sasaki
Dec 19, 2016·Molecular and Cellular Neurosciences·Benedetta Elena FornasariGiovanna Gambarotta

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Citations

Jan 27, 2019·Journal of Molecular Neuroscience : MN·Jessica KronenbergMartin Stangel
Jul 26, 2021·European Journal of Pharmacology·Jiang-Nan SunLi-Ying Qiu

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Methods Mentioned

BETA
PCR

Software Mentioned

SPSS

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