The effects of neuronal induction on gene expression profile in bone marrow stromal cells (BMSC)--a preliminary study using microarray analysis

Brain Research
Satoshi YamaguchiYoshinobu Iwasaki

Abstract

Bone marrow stromal cells (BMSC) have been anticipated as a donor for cell type for transplantation therapy in various neurological disorders. However, their neurogenic capacity still remains undetermined. In this study, we aimed to clarify whether in vitro chemical treatment promotes their neuronal differentiation on the level of gene expression. Mice BMSC were cultured with medium supplemented with DMSO, retinoic acid, and basic fibroblast growth factor, and their morphology and expression of neuronal markers were evaluated. Subsequently, using microarray and RT-PCR techniques, the treatment-induced changes in the gene expression profile were analyzed. After exposure to the medium, the BMSC simulated a neuron-like appearance and increased their immunoreactivity for nestin and Tuj-1. Microarray analysis revealed that the BMSC per se express the multilineage cellular genes, including those associated with the neuron. Chemical treatment significantly decreased the expression of genes related to mesenchymal cells and increased the expression of 5 neuron-associated genes. Microarray and RT-PCR analyses also demonstrated that the BMSC express the genes for several growth factors including NGF-beta and BDNF, indicating their therape...Continue Reading

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Citations

Nov 11, 2008·Neuropathology : Official Journal of the Japanese Society of Neuropathology·Hiroyuki ItosakaYoshinobu Iwasaki
Jan 28, 2009·Neuropathology : Official Journal of the Japanese Society of Neuropathology·Katsuhiko MaruichiYoshinobu Iwasaki
Sep 10, 2009·Neuropathology : Official Journal of the Japanese Society of Neuropathology·Hideo ShichinoheYoshinobu Iwasaki
Oct 23, 2009·Neuropathology : Official Journal of the Japanese Society of Neuropathology·Weidong GuBuwei Yu
Nov 21, 2009·Neuropathology : Official Journal of the Japanese Society of Neuropathology·Yasuhiro ChibaYoshinobu Iwasaki
Jun 26, 2012·Neuropathology : Official Journal of the Japanese Society of Neuropathology·Masahito KawaboriNagara Tamaki
Jan 15, 2014·World Journal of Gastroenterology : WJG·Hua-Lian Hang, Qiang Xia
May 9, 2009·Neurological Research·Mohammad Taghi GhorbanianYaghoub Fathollahi
Nov 26, 2011·The International Journal of Neuroscience·Gholam Reza KakaHadi Kazemi
Oct 18, 2011·Artificial Cells, Blood Substitutes, and Immobilization Biotechnology·Hua JiaXiao-Hong Sun
Jan 19, 2012·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Koji AbeNobutaka Kawahara
Mar 18, 2016·Neurologia Medico-chirurgica·Satoshi Kuroda
Mar 19, 2014·Neurorehabilitation and Neural Repair·Hideo ShichinoheSatoshi Kuroda
Nov 22, 2007·Journal of Neuroscience Research·Masaaki HokariYoshinobu Iwasaki
Jun 15, 2010·Tsitologiia·A V ShakhbazovM P Potapnev
Jul 5, 2011·Critical Care Medicine·Elisa R ZanierMaria-Grazia De Simoni
Feb 22, 2011·Journal of Tissue Engineering and Regenerative Medicine·Bong-Wook ParkGyu-Jin Rho

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