Abstract
A number of diverse biological compounds involved in the regulation of the hypothalamo-hypophyseal-ovarian axis have been examined for effects on the conversion of 3H-progesterone to 3H-5 alpha-dihydro-progesterone and 3H-3 alpha-hydroxy-5 alpha-pregnan-20-one by female rat hypothalamus and/or anterior pituitary. Broken cell preparations were incubated with 3H-progesterone and NADPH, and product 5 alpha-reduced progestins were quantitated by reverse isotopic dilution analysis. Progesterone 5 alpha-reductase activity was reduced up to 50% in the presence of 10(-2) to 10(-3) M serotonin in both preparations. At 10(-3) M, various indoles including n-acetylserotonin, melatonin, 5-methoxytryptamine, 5-methoxytryptophol, and 5-hydroxyindole acetic acid decreased by 10 to 30% 5 alpha-reduced product formation. At 10(-2) M, carbamylcholine and norepinephrine were without effect, while 10(-2) M dopamine reduced by 20% the 5 alpha-reduction of progesterone only in pituitary homogenates. The LHRH protease inhibitor bacitracin (2 X 10(-3) M) decreased by 10 to 40% progesterone 5 alpha-reductase activity in both tissues. By itself, LHRH did not affect the 5 alpha-reduction of progesterone nor did it potentiate the bacitracin effect. In the ...Continue Reading
References
Feb 1, 1976·Endocrinology·P G Harms McCann
Apr 1, 1977·Physiological Reviews·J A Nathanson
Mar 11, 1977·Annals of the New York Academy of Sciences·H H Feder, B L Marrone
Mar 1, 1977·Endocrinology·K M Nuti, H J Karavolas
Jun 1, 1977·Endocrinology·L W Kao, J Weisz
Oct 1, 1977·Endocrinology·C A Blake
Mar 1, 1979·Endocrinology·S R OjedaS M McCann
Jun 1, 1979·Biology of Reproduction·L V DePaolo, C A Barraclough
Jul 1, 1979·Journal of Steroid Biochemistry·M Zanisi, L Martini
Jul 1, 1979·Journal of Steroid Biochemistry·H J KaravolasD J O'Brien
Dec 1, 1977·Hormones and Behavior·C Kubli-Garfias, R E Whalen
Jun 1, 1978·Steroids·J E Krause, H J Karavolas
Dec 2, 1976·Nature·D K SarkarN M Sherwood
Nov 22, 1976·Biochemical and Biophysical Research Communications·J F McKelvyC Kordon
Jan 1, 1977·Neuroendocrinology·T F Ogle, J I Kitay
Oct 1, 1976·Steroids·F V NowakH J Karavolas
Mar 15, 1975·Life Sciences·S M McCann, R L Moss
Jan 1, 1975·Neuroendocrinology·B Has Milković
Aug 1, 1972·The Journal of Endocrinology·A P Labhsetwar
Jan 1, 1974·Journal of Reproduction and Fertility·B N SridharanH J Karavolas
Mar 1, 1974·The Journal of Endocrinology·J L FrehnL C Ellis
Jul 24, 1974·Biochemical and Biophysical Research Communications·G V MarinettiR Love
Apr 1, 1971·Endocrinology·I A KamberiJ C Porter
Jan 1, 1969·Neuroendocrinology·C Kordon
Feb 1, 1980·Endocrinology·F KimuraS M McCann
Jun 1, 1964·Endocrinology·W K O'STEEN