The effects of oral glutamine on cyclophosphamide-induced nephrotoxicity in rats

Human & Experimental Toxicology
Premila Abraham, Bina Isaac

Abstract

Nephrotoxicity is one of the adverse side effects of cyclophosphamide (CP) chemotherapy. In a recent study, we have demonstrated that oxidative stress and glutathione depletion play important roles in CP-induced renal damage. The aim of the study was to verify whether glutamine, the precursor for glutathione synthesis, prevents CP-induced oxidative stress and renal damage using a rat model. Adult male rats were administered a single dose of 150 mg/ kg body weight of CP intraperitoneally. The glutamine-pretreated rats were administered 1 gm/kg body weight of glutamine orally 2 h before the administration of CP. Vehicle/glutamine-treated rats served as controls. All the rats were killed 16 h after the dose of CP/vehicle. The kidneys were removed and used for light microscopic and biochemical studies. The markers of oxidative stress including malondialdehyde content, protein carbonyl content, protein thiol, reduced glutathione and myeloperoxidase activity, a marker of neutrophil infiltration, were measured in kidney homogenates. CP treatment-induced damage to kidney involved the glomeruli and the tubules. Pretreatment with glutamine reduced CP-induced glutathione depletion and increased myeloperoxidase activity. However, it did no...Continue Reading

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Citations

Jul 25, 2013·Journal of the Formosan Medical Association = Taiwan Yi Zhi·Ching-Chia WangShing-Hwa Liu
Dec 24, 2015·Canadian Journal of Physiology and Pharmacology·Khaled A AlhumaidhaHelmy Moawad
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Jul 12, 2017·Fundamental & Clinical Pharmacology·Mehdi GoudarziSaeed Mehrzadi
Feb 17, 2021·Clinical and Experimental Pharmacology & Physiology·Hanaa WanasBasma Emad Aboulhoda
Nov 12, 2020·Canadian Journal of Physiology and Pharmacology·Arthur H SousaCarlos R Tirapelli
Nov 4, 2021·BMC Complementary Medicine and Therapies·Quadri K AlabiDeborah Kajewole-Alabi

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