The effects of pantothenate deficiency and acetate addition on anaerobic batch fermentation of glucose by Saccharomyces cerevisiae

Applied Microbiology and Biotechnology
M J TaherzadehC Niklasson

Abstract

Physiological effects of deficiency of pantothenate, a necessary precursor in the synthesis of coenzyme A, were studied using the yeast strain Saccharomyces cerevisiae CBS 8066. Cells were grown on defined media in anaerobic batch cultures with glucose (50 g/l) as the carbon and energy source. Batch cultures containing more than 60 micrograms/l pantothenate showed no significant differences with respect to growth rates and product yields. However, with an initial pantothenate concentration at 30 micrograms/l, the average glucose consumption rate was 50% lower than in rich medium and, at even lower concentration of pantothenate, the culture did not consume all the glucose in the medium. Furthermore, pantothenate deficiency caused the acetate and pyruvate yields to increase and the biomass yield to decrease, compared to the yields in pantothenate-rich medium. The increased acetate formation could be counteracted by initial addition of acetate to the medium, and thereby the glycerol yield could be decreased. An initial addition of acetate of 1.6 g/l to pantothenate-deficient medium (30 micrograms/l) caused a 35% decrease in glycerol yield and a 6% increase in ethanol yield. Furthermore, the time required for complete conversion of...Continue Reading

Citations

Jan 16, 2007·Applied Microbiology and Biotechnology·Tobias ModigGunnar Lidén
Nov 9, 2006·Biotechnology Letters·Anneli Petersson, Gunnar Lidén
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Jul 4, 2003·Applied and Environmental Microbiology·Ilona Sárvári HorváthGunnar Lidén
Oct 31, 2012·International Journal of Molecular Sciences·Johan O WestmanMohammad J Taherzadeh
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Dec 18, 2001·Biotechnology and Bioengineering·I S HorváthG Lidén
Nov 18, 2010·Journal of Bioscience and Bioengineering·Cristhian CarrascoGunnar Lidén
Jan 1, 2014·Biotechnology Progress·Yogender Kumar GowthamSarah W Harcum
Oct 7, 2004·Journal of Biotechnology·Ronny PurwadiMohammad J Taherzadeh
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Mar 18, 2005·Biotechnology and Bioengineering·Farid TalebniaMohammad J Taherzadeh
Apr 29, 2008·Biotechnology and Bioengineering·Tobias ModigGunnar Lidén

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