The effects of particulate cobalt, chromium and cobalt-chromium alloy on human osteoblast-like cells in vitro

The Journal of Bone and Joint Surgery. British Volume
M J AllenN Rushton

Abstract

Particulate wear debris can induce the release of bone-resorbing cytokines from cultured macrophages and fibroblasts in vitro, and these mediators are believed to be the cause of the periprosthetic bone resorption which leads to aseptic loosening in vivo. Much less is known about the effects of particulate debris on the growth and metabolism of osteoblastic cells. We exposed two human osteoblast-like cell lines (SaOS-2 and MG-63) to particulate cobalt, chromium and cobalt-chromium alloy at concentrations of 0, 0.01, 0.1 and 1.0 mg/ml. Cobalt was toxic to both cell lines and inhibited the production of type-I collagen, osteocalcin and alkaline phosphatase. Chromium and cobalt-chromium were well tolerated by both cell lines, producing no cytotoxicity and no inhibition of type-I collagen synthesis. At the highest concentration tested (1.0 mg/ml), however, chromium inhibited alkaline phosphatase activity, and both chromium and cobalt-chromium alloy inhibited osteocalcin expression. Our results clearly show that particulate metal debris can modulate the growth and metabolism of osteoblastic cells in vitro. Reduced osteoblastic activity at the bone-implant interface may be an important mechanism by which particulate wear debris influ...Continue Reading

Citations

Oct 18, 2007·Archives of Orthopaedic and Trauma Surgery·Martina MarkerRainer Kotz
Jun 14, 2005·The Orthopedic Clinics of North America·J C Le HuecT Friesem
Aug 28, 2001·Journal of Biomechanics·P J FirkinsJ Fisher
Feb 27, 2004·Medical Engineering & Physics·E ChassotG Vanneuville
Nov 23, 2010·International Orthopaedics·Martin Pospischill, Karl Knahr
Dec 17, 2008·Acta Orthopaedica·Guillaume MabilleauAfsie Sabokbar
Jun 12, 2010·Chest·Valérie GounantJocelyne Fleury-Feith
Jan 23, 1999·Journal of Orthopaedic Science : Official Journal of the Japanese Orthopaedic Association·T TsutsuiT Nakamura
Apr 6, 2001·Journal of Orthopaedic Science : Official Journal of the Japanese Orthopaedic Association·T HirayamaT Torisu
Oct 16, 2003·Spine·Paul A AndersonCathy S Carlson
Sep 15, 2004·Veterinary Surgery : VS·Alexander O El-WarrakBrigitte von Rechenberg
Apr 24, 2008·Journal of Biomedical Materials Research. Part a·Robert LenzRainer Bader
May 23, 2003·Journal of Biomedical Materials Research. Part a·Stuart B GoodmanR Lane Smith
Jan 6, 2004·Journal of Biomedical Materials Research. Part a·Akiko YamamotoTakao Hanawa
Nov 23, 2006·Journal of Biomedical Materials Research. Part a·Wen-Hsi WangChun-Pin Lin
Jul 4, 2007·Journal of Biomedical Materials Research. Part a·Gema VallésNuria Vilaboa
Jul 20, 2006·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·J L González-CarrascoN Baldini
Feb 5, 2009·Journal of Biomedical Materials Research. Part a·Petra GundePeter J Uggowitzer
Dec 13, 2006·The Journal of Arthroplasty·Shu SaitoKaichiro Saigo
Oct 26, 2005·Biomaterials·Mark P StaigerGeorge Dias
May 2, 2015·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Karan M ShahAlison Gartland
Oct 7, 2014·Knee Surgery, Sports Traumatology, Arthroscopy : Official Journal of the ESSKA·Ruud P van HovePeter A Nolte
Jul 13, 2012·The Journal of Arthroplasty·Romain DesmarchelierMichel-Henri Fessy
May 16, 2014·Toxicology and Applied Pharmacology·Leah J SmithJohn Pierce Wise
Apr 5, 2011·Orthopaedics & Traumatology, Surgery & Research : OTSR·J-M LaffosseP-A Vendittoli
Nov 30, 2014·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Olga M PosadaM Helen Grant
Nov 16, 2004·The Spine Journal : Official Journal of the North American Spine Society·Paul A AndersonJan Goffin
Mar 1, 2017·Basic & Clinical Pharmacology & Toxicology·Mona KumantoEeva Moilanen
Jan 22, 2005·Clinical Orthopaedics and Related Research·Mauricio SilvaThomas P Schmalzried
Jul 27, 2006·Human & Experimental Toxicology·S SankaramanivelN Srinivasan
Jun 12, 2003·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Mark L WangRocky S Tuan

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