PMID: 7011807Feb 1, 1981Paper

The effects of pH and redox potential on the hydrogen production activity of the hydrogenase from Megasphaera elsdenii

European Journal of Biochemistry
C Van Dijk, C Veeger

Abstract

The effects of temperature on the ionization constant (pK') and apparent midpoint potential (EB) of the unprotonated species of Megasphaera elsdenii flavodoxin hydroquinone shows that, above 15 degrees C, delta pK' . K-1 = -9.7 X 10(-3) and delta EB . K-1 = -0.6 mV. The effects of pH and redox potential on the hydrogen production activity with fixed concentrations of methyl viologen semiquinone (0.3 mM; artificial donor) and M. elsdenii flavodoxin hydroquinone (50 microM; natural donor) show that with decreasing pH the activity increases. Irrespective of the pH and electron donor, at increasing redox potential, a redox-potential-independent production activity is followed by a redox-potential-dependent production activity. This redox-potential-dependent behaviour of the hydrogen production activity represents an n = 2-type of redox titration curve with an 'apparent midpoint potential' which corresponds with the potential of the hydrogen electrode at that pH. The effect of pH on the manometrically determined hydrogen production activity (direct) is in good agreement with that determined spectrophotometrically (indirect; see preceding paper), with both electron donors tested. In contrast to predictions from the models for hydroge...Continue Reading

References

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Jan 1, 1980·European Journal of Biochemistry·C van DijkC Veeger

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Citations

Mar 1, 1983·European Journal of Biochemistry·H J GrandeC Veeger
Apr 1, 1992·European Journal of Biochemistry·G M Doherty, S G Mayhew
Aug 13, 2013·Nature Chemical Biology·Julian EsselbornThomas Happe
Sep 2, 2015·Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering·Jeonghee YunKyung-Suk Cho
Jan 21, 2018·Journal of Animal Physiology and Animal Nutrition·Y HuangC Bayourthe
Sep 12, 2007·Chemical Reviews·Wolfgang LubitzMaurice van Gastel
Jun 29, 1983·Biochimica Et Biophysica Acta·M PinkwartH G Schlegel
Jan 1, 1989·Archives of Biochemistry and Biophysics·R R PetrovV O Popov
Feb 1, 1982·Analytical Biochemistry·V M FernándezA Ballesteros
Jan 30, 1987·Biochemical and Biophysical Research Communications·I B UtkinI V Berezin
Jan 1, 1986·Biochimie·A van Berkel-ArtsC Veeger

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