The effects of plant extracts on microbial community structure in a rumen-simulating continuous-culture system as revealed by molecular profiling.

Folia Microbiologica
D FermeG Avgustin

Abstract

An in vitro study in dual-flow continuous-culture fermentors was conducted with two different concentrations of monensin, cinnamaldehyde or garlic extract added to 1:1 forage-to-concentrate diet in order to determine their effects on selected rumen bacterial populations. Samples were subjected to total DNA extraction, restriction analysis of PCR amplified parts of 16S rRNA genes (ARDRA) and subsequent analysis of the restriction profiles by lab-on-chip technology with the Agilent's Bioanalyser 2100. Eub338-BacPre primer pair was used to select for the bacteria from the genera Bacteroides, Porphyromonas and Prevotella, especially the latter representing the dominant Gram-negative bacterial population in the rumen. Preliminary results of HaeIII restriction analysis show that the effects of monensin, cinnamaldehyde and garlic extract on the BacPre targeted ruminal bacteria are somewhat different in regard to targeted populations and to the nature of the effect. Garlic extract was found to trigger the most intensive changes in the structure of the BacPre targeted population. Comparison of the in silico restriction analysis of BacPre sequences deposited in different DNA databanks and of the results of performed amplified ribosomal D...Continue Reading

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Citations

Jan 18, 2006·Folia Microbiologica·K MihalikováS Kisidayová
Sep 30, 2006·Folia Microbiologica·K HorosováV Kmet
Sep 30, 2006·Folia Microbiologica·J MrázekJ Kopecný
Jul 8, 2009·Antonie van Leeuwenhoek·Amlan K Patra, Jyotisna Saxena
Apr 25, 2012·Folia Microbiologica·Darja Kušar, Gorazd Avguštin
Jul 1, 2010·Animal : an International Journal of Animal Bioscience·S CalsamigliaA M van Vuuren
Nov 2, 2014·Asian-Australasian Journal of Animal Sciences·Sangbuem ChoNag-Jin Choi
May 23, 2007·Journal of Dairy Science·S CalsamigliaA Ferret
May 7, 2005·Journal of Dairy Science·A BachM D Stern
Nov 18, 2005·Journal of Dairy Science·M BusquetC Kamel
Aug 1, 2018·Veterinary Research·Hyun LillehojCyril G Gay
May 1, 2021·Animals : an Open Access Journal From MDPI·Rokia TemmarSergio Calsamiglia

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