The effects of platelet-derived growth factor-BB on periodontal cells in an in vitro wound model

Journal of Periodontology
J H MumfordT W Oates

Abstract

Platelet-derived growth factor (PDGF-BB) has been shown to enhance periodontal regeneration. Principles of guided tissue regeneration dictate that one of the goals of therapy is to modulate the wound healing processes to favor repopulation of the wound with cells derived from the periodontal ligament rather than from the gingival tissues. Using an in vitro wound model, gingival fibroblasts (GF) have been shown to fill a wound space significantly faster than periodontal ligament cells (PDL). There are no data reported directly comparing the response of these 2 cell types to PDGF-BB within such a wound model. Therefore, the aims of this research were: 1) to characterize both the proliferative and wound fill (WF) effects of PDGF-BB within an in vitro model and 2) to compare specific growth factor effects between GF and PDL. Primary cultures of both human PDL and GF were derived from explanted tissues and passaged to 12-well tissue culture plates. Triplicate cultures of both cell types were grown to confluence and in vitro wounds were mechanically created, removing a 3 mm wide band of the cell layer across the diameter of the wells. The wells were then incubated for 2, 6, and 9 days in media containing 0.1% fetal bovine serum (FBS)...Continue Reading

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Citations

Sep 19, 2003·Tissue Engineering·Orasa AnusaksathienWilliam V Giannobile
Sep 30, 2003·Journal of Periodontology·Xiaozhe Han, Salomon Amar
Dec 7, 2007·Journal of Periodontology·Adriana C P Sant'AnaMaria Lúcia Rubo de Rezende
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Nov 5, 2011·Dental Traumatology : Official Publication of International Association for Dental Traumatology·Katsuya NodaAtsushi Saito
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Feb 24, 2015·Dental Materials : Official Publication of the Academy of Dental Materials·Sophia P PilipchukWilliam V Giannobile
Nov 15, 2014·PloS One·Hermann AgisWilliam V Giannobile
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Jul 10, 2017·ACS Biomaterials Science & Engineering·Pedro S BaboManuela E Gomes

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