The effects of poly L-lactic acid nanofiber scaffold on mouse spermatogonial stem cell culture

International Journal of Nanomedicine
Neda EslahiMorteza Koruji

Abstract

A 3D-nanofiber scaffold acts in a similar way to the extracellular matrix (ECM)/basement membrane that enhances the proliferation and self-renewal of stem cells. The goal of the present study was to investigate the effects of a poly L-lactic acid (PLLA) nanofiber scaffold on frozen-thawed neonate mouse spermatogonial stem cells (SSCs) and testis tissues. The isolated spermatogonial cells were divided into six culture groups: (1) fresh spermatogonial cells, (2) fresh spermatogonial cells seeded onto PLLA, (3) frozen-thawed spermatogonial cells, (4) frozen-thawed spermatogonial cells seeded onto PLLA, (5) spermatogonial cells obtained from frozen-thawed testis tissue, and (6) spermatogonial cells obtained from frozen-thawed testis tissue seeded onto PLLA. Spermatogonial cells and testis fragments were cryopreserved and cultured for 3 weeks. Cluster assay was performed during the culture. The presence of spermatogonial cells in the culture was determined by a reverse transcriptase polymerase chain reaction for spermatogonial markers (Oct4, GFRα-1, PLZF, Mvh(VASA), Itgα6, and Itgβ1), as well as the ultrastructural study of cell clusters and SSCs transplantation to a recipient azoospermic mouse. The significance of the data was anal...Continue Reading

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Citations

Apr 8, 2016·Journal of Assisted Reproduction and Genetics·Saeid AzizollahiMorteza Koruji
Apr 17, 2016·Journal of Ethnopharmacology·Maryam MahaldashtianSeyedeh Momeneh Mohamadi
Apr 24, 2021·Journal of Materials Science. Materials in Medicine·Fatemeh AsgariMorteza Koruji

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Methods Mentioned

BETA
FCS
PCR
electrophoresis
PCRs
light
transmission electron microscopy
xenografting

Software Mentioned

Gene Runner
UVItec
Image J

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