The effects of pre-analysis sample handling on human plasma amino acid concentrations

Clinica Chimica Acta; International Journal of Clinical Chemistry
Shunji TakehanaHiroshi Miyano

Abstract

The accurate and reliable quantification of amino acid concentrations in human plasma is important for the investigation of a number of diseases. However, few systematic studies investigating the changes in amino acid concentrations related to blood collection and storage conditions have been completed. Blood samples were collected with EDTA-Na2 from 3 healthy volunteers and subjected to a number of different treatments; hemolysis, temperature after blood collection, time from blood collection to cooling, the influence of platelets, long term storage conditions, and repeated freeze-thaw cycles. Changes in the concentrations of 22 amino acids were determined using an Amino Acid Analyzer. Of the conditions influencing sample stability between blood collection and amino acid analysis, hemolysis, temperature after blood collection, and long-term storage at -20°C affected the concentrations of 11 amino acids. Time from blood collection to cooling, platelet contamination and repeated freeze-thaw cycles altered the levels of 4 amino acids. We observed changes in amino acid concentrations relating to blood collection and storage conditions. If attention is paid to 4 key factors (hemolysis, temperature immediately following blood collec...Continue Reading

References

Jun 14, 1985·Clinica Chimica Acta; International Journal of Clinical Chemistry·S Sahai, S Uhlhaas
Jul 1, 1969·Clinica Chimica Acta; International Journal of Clinical Chemistry·T L Perry, S Hansen
Mar 1, 1970·Clinica Chimica Acta; International Journal of Clinical Chemistry·G A Young, F M Parsons
Aug 24, 1999·Current Opinion in Clinical Nutrition and Metabolic Care·E HolmE Grips
Dec 1, 1994·Clinical Nutrition : Official Journal of the European Society of Parenteral and Enteral Nutrition·H M Van EijkP B Soeters
Jan 28, 2009·Journal of Agricultural and Food Chemistry·Hiroo YoshidaHiroshi Miyano
Jul 18, 2015·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Hiroo YoshidaHiroshi Miyano

❮ Previous
Next ❯

Citations

Jul 6, 2016·Current Opinion in Clinical Nutrition and Metabolic Care·Kenji Nagao, Minoru Yamakado
Jul 31, 2018·Expert Review of Proteomics·Viviana GrecoAndrea Urbani
Apr 5, 2020·Orphanet Journal of Rare Diseases·Kimber van VlietM Rebecca Heiner-Fokkema
May 21, 2020·Biopreservation and Biobanking·Katrin FreiburghausCarlo R Largiadèr
Dec 2, 2020·Nutrition Reviews·Kenji Nagao, Takeshi Kimura
Apr 4, 2021·Research in Veterinary Science·Chih-Fan ChiangAndrea J Fascetti
Dec 15, 2020·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Ivonne McM RietjensKeng Ngee Teoh

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.