The Effects of Prednisolone Treatment on Cytokine Expression in Patients with Erythema Nodosum Leprosum Reactions

Frontiers in Immunology
Edessa NegeraDiana N J Lockwood

Abstract

Erythema nodosum leprosum (ENL) is a systemic inflammatory complication occurring mainly in patients with lepromatous leprosy (LL) and borderline lepromatous leprosy. Prednisolone is widely used for treatment of ENL reactions but clinical improvement varies. However, there is little good in vivo data as to the effect of prednisolone treatment on the pro-inflammatory cytokines in patients with ENL reactions. As a result, treatment and management of reactional and post-reactional episodes of ENL often pose a therapeutic challenge. We investigated the effect of prednisolone treatment on the inflammatory cytokines TNF, IFN-γ, IL-1β, IL-6, and IL-17 and the regulatory cytokines IL-10 and TGF-β in the skin lesion and blood of patients with ENL and compared with non-reactional LL patient controls. A case-control study was employed to recruit 30 patients with ENL and 30 non-reactional LL patient controls at ALERT Hospital, Ethiopia. Blood and skin biopsy samples were obtained from each patient before and after prednisolone treatment. Peripheral blood mononuclear cells from patients with ENL cases and LL controls were cultured with M. leprae whole-cell sonicates (MLWCS), phytohemagglutinin or no stimulation for 6 days. The supernatants ...Continue Reading

References

Apr 1, 1992·Infection and Immunity·P F BarnesR L Modlin
Sep 26, 1994·Neuroscience Letters·W A BanksE G Gutierrez
Jan 1, 1996·Advances in Immunology·A Billiau
Aug 1, 1998·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·E P Sampaio, E N Sarno
Nov 5, 1998·International Journal of Dermatology·A D MoubasherD D Raheem
Nov 7, 2002·Archives of Dermatological Research·Rosane M B TelesElizabeth P Sampaio
Mar 9, 2004·Proceedings of the National Academy of Sciences of the United States of America·Javier Cote-SierraWilliam E Paul
May 24, 2005·Infection and Immunity·Anna K AnderssonDiana N J Lockwood
Jun 29, 2005·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Stefan Ehlers
Nov 17, 2005·The Journal of Infectious Diseases·Patrick A J HaslettGilla Kaplan
Sep 18, 2008·Indian Journal of Dermatology, Venereology and Leprology·K H MohanSmitha Prabhu
May 29, 2009·BMC Infectious Diseases·Mariane M StefaniDavid M Scollard
Feb 8, 2011·Biochimica Et Biophysica Acta·Jürgen SchellerStefan Rose-John
Dec 20, 2011·PLoS Neglected Tropical Diseases·Diana N J LockwoodSujai Suneetha
Mar 31, 2012·The Journal of Infectious Diseases·Ana Lucia M SousaMariane M A Stefani
Mar 15, 2014·PLoS Neglected Tropical Diseases·Stephen L WalkerSaba M Lambert
Apr 12, 2014·PLoS Neglected Tropical Diseases·Kidist BoboshaAnnemieke Geluk
Nov 30, 2014·Clinics in Dermatology·Indira NathVijaya Lakshmi Valluri
Sep 1, 2013·Emerging Microbes & Infections·Wei Jin, Chen Dong
Oct 11, 2017·PLoS Neglected Tropical Diseases·Edessa NegeraDiana N Lockwood

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Citations

Oct 2, 2020·Current Opinion in Ophthalmology·Sivaraman Bala MuruganYogish Kamath

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Methods Mentioned

BETA
biopsies
Biopsy
density gradient centrifugation
enzyme-linked immunosorbent assay
ELISA
electrophoresis
Polymerase Chain Reaction
PCR

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