The effects of prostaglandin E2 on DNA and collagen synthesis in osteoblasts in vitro

Calcified Tissue International
M Nagai

Abstract

The effects of prostaglandin E2(PGE2) on DNA and collagen synthesis in two separate cell populations were investigated. In view of their morphology, ALPase activity, DNA and collagen synthesis, and response to PGE2, one population was in an undifferentiated state consisting of preosteoblast-like (PL) cells and the other was in a differentiated state consisting predominantly of osteoblast-like (OB) cells. As parameters of bone-forming activity, the incorporation of 3H-thymidine into DNA and the incorporation of 3H-proline into collagenase digestible protein were measured to assess DNA and collagen synthesis. The cells were treated with PGE2 in the presence of indomethacin (IM) to avoid the influence of endogenous prostaglandins. At 24 hours, IM stimulated the DNA synthesis in both cell populations. Furthermore, a greater stimulation was found in the PL cells than in the OB cells. On the other hand, exogenously supplemented PGE2 reversed the IM-induced stimulation of DNA synthesis. In contrast, high concentrations of PGE2 alone increased the DNA synthesis. With respect to collagen synthesis, IM showed an inhibitory effect, especially in the PL cells. This inhibitory effect was also reversed by the addition of PGE2. In addition to...Continue Reading

References

Dec 6, 1976·Biochemical and Biophysical Research Communications·C V ByusD H Russell
Jun 28, 1985·Biochemical and Biophysical Research Communications·A van der PlasP J Nijweide
Sep 1, 1985·Prostaglandins·N C PartridgeT J Martin
Dec 10, 1974·Prostaglandins·L G Raisz, A R Koolemans-Beynen
Oct 25, 1972·Nature: New Biology·N Blumenkrantz, J Sondergaard
Jun 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·M K HaddoxD H Russell
May 1, 1983·Calcified Tissue International·R D NolanT J Martin
Jan 1, 1984·Prostaglandins·Y S Chyun, L G Raisz
Oct 30, 1981·Biochemical and Biophysical Research Communications·S B RodanL G Raisz
Jan 1, 1981·Anatomy and Embryology·G VolpiM A Muglia
Nov 1, 1980·The Journal of Pediatrics·K SoneT Kuroume

❮ Previous
Next ❯

Citations

Dec 1, 1991·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·S C RawlinsonL E Lanyon
Mar 1, 1995·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·A Scutt, P Bertram
Oct 1, 1995·Diabetes Research and Clinical Practice·Y YamadaT Kasama
Nov 1, 1992·Cellular Signalling·B OgisoC A McCulloch
Feb 1, 1997·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·L Y TangD B Kimmel
Feb 1, 1996·Calcified Tissue International·G P Thomas, A J el Haj
Jan 1, 1995·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·K IjiriT Shimizu
Apr 1, 1994·Prostaglandins, Leukotrienes, and Essential Fatty Acids·K IgarashiH Mitani
Apr 16, 2013·Archives of Oral Biology·Polbhat TripuwabhrutKamal Mustafa
Feb 1, 1996·Prostaglandins, Leukotrienes, and Essential Fatty Acids·M A FangS McDougall
Mar 21, 2002·Expert Opinion on Therapeutic Targets·J H TobiasA M Scutt
Nov 1, 1995·Calcified Tissue International·R L Duncan, C H Turner
Apr 1, 1997·Environmental Health Perspectives·N T TelangM P Osborne
Sep 1, 1991·The American Journal of Physiology·K M Reich, J A Frangos

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.