The effects of reduced oxygen tension on cell proliferation and matrix synthesis in synovium and tendon explants from the rabbit carpal tunnel: an experimental study in vitro

Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society
D Rempel, S O Abrahamsson

Abstract

Local ischemia may play an important role in the development of tendon degeneration as well as entrapment neuropathies. In order to investigate the cellular effects of hypoxia on tendon and synovial tissue from the carpal canal, dose response effects of oxygen on cell proliferation and synthesis of matrix components were examined in segments of synovial and flexor digitorum profundus tendon from the carpal tunnel of rabbits during short term culture. Explants were incubated in airtight containers flushed with either 0%, 1%, 3%, 20% O2 plus 2% CO2 and N2 to balance and labeled with either 3H-thymidine or 3H-proline and 35S-sulfate. Cell proliferation was significantly inhibited by hypoxia in synovium but not in tendon (P = 0.03). In parallel, the synthesis of non-collagenous proteins was significantly reduced in synovium but not in tendon (P = 0.006). In both tissues hypoxia significantly inhibited collagen synthesis. On the other hand, hypoxia had no significant effect on the synthesis of new proteoglycans as determined by 35S-sulfate incorporation. Hypoxia can inhibit cell proliferation and alter synthesis of matrix components in synovial tissue, but may only have minor effects on non-collagen protein synthesis in tendon expla...Continue Reading

References

Jan 1, 1977·Scandinavian Journal of Plastic and Reconstructive Surgery·B Rydevik, G Lundborg
Nov 1, 1991·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·C T BrightonC C Clark
Jul 1, 1991·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·S O AbrahamssonL S Lohmander
Jan 1, 1989·Scandinavian Journal of Plastic and Reconstructive Surgery and Hand Surgery·S O AbrahamssonL S Lohmander
Jan 1, 1988·The Japanese Journal of Physiology·T Takemiya, J Maeda
Jan 1, 1988·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·A J BanesL Dahners
Apr 1, 1987·Annals of the Rheumatic Diseases·M F BrownA H Murley
Jan 1, 1987·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Y OkudaP C Amadio
Jul 1, 1972·Developmental Biology·R F Diegelmann, B Peterkofsky
Sep 9, 1974·Biochemical and Biophysical Research Communications·J Uitto, D J Prockop
Jan 1, 1984·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·D AmielW Akeson
Dec 1, 1983·Journal of Neurology, Neurosurgery, and Psychiatry·G LundborgH Powell
Mar 1, 1994·The Journal of Hand Surgery·J W ArkD Amiel
Mar 1, 1997·Research in Veterinary Science·H L BirchA E Goodship
May 1, 1997·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·D RempelA Hargens
Jan 8, 1998·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·S W O'DriscollC N Commisso

❮ Previous
Next ❯

Citations

Sep 10, 2008·Hand : Official Journal of the American Association for Hand Surgery·Anke M EttemaPeter C Amadio
Mar 2, 2010·The Journal of Bone and Joint Surgery. British Volume·R T BensonP A Hulley
May 17, 2012·Orthopedics·Andreas F MavrogenisPanayiotis J Papagelopoulos
Jul 1, 2010·Journal of Thrombosis and Haemostasis : JTH·L A Valentino
Aug 12, 2008·Human Factors·David Rempel
Feb 24, 2007·Human Factors·Peter J KeirDavid M Rempel
May 25, 2005·The Journal of Bone and Joint Surgery. British Volume·M J OddyG W Blunn
Jul 19, 2012·Journal of Surgical Oncology·Andreas F MavrogenisPietro Ruggieri
Mar 9, 2005·Journal of Cellular Physiology·David W WangFarshid Guilak
Sep 6, 2005·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Alexander ScottVincent Duronio
Jun 17, 2006·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Eeva RissanenMikko Nikinmaa
Sep 1, 2006·The Journal of Bone and Joint Surgery. British Volume·C J PendegrassG W Blunn
Mar 11, 2003·Journal of Applied Physiology·A CogoJ G Martin
Jan 19, 2021·Oxidative Medicine and Cellular Longevity·Chen ChenYa Fang Wu
May 16, 2009·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Dorthe SkovgaardAndreas Kjaer
Oct 27, 2020·ACS Biomaterials Science & Engineering·Rattapol PinnaratipBruce P Lee

❮ Previous
Next ❯

Related Concepts

Related Feeds

Autoimmune Lymphoproliferative Syndrome

Autoimmune lymphoproliferative syndrome (ALPS) is a rare genetic disorder of abnormal lymphocyte survival caused by defective Fas mediated apoptosis. Discover the latest research on ALPS here.