The effects of siRNA against RPL22 on ET-1-induced proliferation of human pulmonary arterial smooth muscle cells

International Journal of Molecular Medicine
Kai SunHong Wang

Abstract

The aim of this study was to investigate the effects of small interference RNA (siRNA) against ribosomal protein L22 (RPL22) on ET-1-induced proliferation of human pulmonary arterial smooth muscle cells (HPASMCs). HPASMCs were transfected with siRNA against RPL22, incubated in smooth muscle cell culture medium (SMCM) and ET-1. RPL22 gene expression and protein levels of HPASMCs were measured by real-time PCR and western blotting, respectively. PCNA, CCK-8 immunohistochemistry and flow cytometry analysis were used to evaluate HPASMC proliferation. Cyclin D1 (CCND1) expression was also assayed. Following transfection with siRNA against RPL22, RPL22 expression was significantly inhibited in the control and ET-1 groups. HPASMC proliferation was significantly suppressed by transfection with siRNA against RPL22. Moreover, CCND1 was also downregulated by inhibiting RPL22 expression. In conclusion, these data suggest that inhibition of RPL22 expression can suppress HPASMC proliferation and CCND1 expression. The effects of siRNA against RPL22 on HPASMC proliferation are considered to be mediated through inhibition of CCND1 expression.

References

Feb 7, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·L Taraseviciene-StewartR M Tuder
Jan 31, 2002·American Journal of Respiratory and Critical Care Medicine·Neil DavieJohn Wharton
Mar 22, 2003·Current Opinion in Cell Biology·Dennis W Stacey
Jan 22, 2004·Circulation·Rohit BudhirajaPaul M Hassoun
Jun 15, 2004·Journal of the American College of Cardiology·Marc HumbertMarlene Rabinovitch
Oct 4, 2005·The Journal of Clinical Investigation·Ralph Theo SchermulyFriedrich Grimminger
Jul 22, 2006·Pediatric Research·Robert P JankovA Keith Tanswell
Jul 28, 2006·European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery·Emin Gurbanov, Xiao Shiliang
Oct 13, 2006·American Journal of Physiology. Lung Cellular and Molecular Physiology·Giorgio LagnaAkiko Hata
Jul 22, 2008·Nature Genetics·Kelly A McGowanGregory S Barsh
Feb 4, 2009·Histopathology·Susan Stewart, Doris Rassl
Jul 9, 2009·Journal of the American College of Cardiology·Nicholas W MorrellE Kenneth Weir
Dec 8, 2009·Pflügers Archiv : European journal of physiology·Eric Thorin, David J Webb
Mar 6, 2010·Advances in Experimental Medicine and Biology·Margaret R Maclean, Yvonne Dempsie
Apr 23, 2010·Journal of Cardiovascular Pharmacology·ZhiYuan PanHai Wang
Aug 6, 2011·Nucleus·Danièle Hernandez-Verdun

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Citations

Nov 7, 2012·Gynecologic Oncology·Akiva P NovetskyPaul J Goodfellow
Jun 26, 2013·Medical Oncology·Mingxia YangLei Zhang

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