The effects of soft cryotherapy on conjunctiva and cornea in isolated pig eyes and comparison with standard liquid nitrogen: A pilot ex vivo study.

Veterinary Ophthalmology
Laura BarachettiAntonella Rampazzo

Abstract

To evaluate the immediate effects on cornea and conjunctiva of a cryosurgical method based on the application of a dimethyl ether, propane and isobutene solution (DMEPI, Histofreezer® ), and of Liquid Nitrogen (LN2 ; Cry-Ac Cryogun® ). A total of 52 isolated pig eyes were obtained at the slaughterhouse, preserved for less than one hour in Ringer Lactate and divided into four groups. The first group included eyes treated with two applications of DMEPI in the center of the cornea, the second group eyes treated with DMEPI on the third eyelid's conjunctiva, the third group eyes treated with LN2 on the third eyelid's conjunctiva and the fourth group included eyes treated with LN2 on the central cornea. Each cryogen application (0.8 cm area) lasted 40 seconds. Each group included one (untreated) control eye. The eyes were submitted for routine histopathological evaluation. Histological alterations were recorded and scored with a semiquantitative scoring system. No tissue alteration was detected on the conjunctiva, in any of the groups. Treated corneas showed mild to moderate cytoplasmic vacuolization of epithelial cells, shrinkage and hypereosinophilia of small groups of basal epithelial cells and stromal cleft formation. Soft cryosu...Continue Reading

References

Mar 29, 1977·Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie. Albrecht Von Graefe's Archive for Clinical and Experimental Ophthalmology·S Yokota, W K Waller
Apr 1, 1979·Archives of Internal Medicine·J L Whittle, J H Bates
Jan 1, 1978·The Journal of Small Animal Practice·J S Baxter
Jul 1, 1977·The Journal of Dermatologic Surgery and Oncology·J Farrant, C A Walter
Jan 1, 1988·The Journal of the Society of Occupational Medicine·S P DeaconJ G Bell
Jan 15, 1987·American Journal of Ophthalmology·A A HidayatN A Rao
Feb 1, 1968·Archives of Ophthalmology·H LincoffJ M McLean
May 1, 1980·The Veterinary Clinics of North America. Small Animal Practice·D A Rickards
Nov 1, 1980·The Veterinary Clinics of North America. Small Animal Practice·M D Bryne
Nov 1, 1980·The Veterinary Clinics of North America. Small Animal Practice·H B Seim
Nov 1, 1980·The Veterinary Clinics of North America. Small Animal Practice·S J Withrow
Nov 1, 1980·The Veterinary Clinics of North America. Small Animal Practice·R E Merideth, K N Gelatt
Mar 1, 1981·Archives of Ophthalmology·J R Wood, R L Anderson
Jan 1, 1995·Micron : the International Research and Review Journal for Microscopy·D A Handley
May 1, 1997·Journal of the American Academy of Dermatology·G Lindgren, O Larkö
Oct 27, 1998·Cryobiology·A A Gage, J Baust
Jun 18, 1999·American Journal of Ophthalmology·S P Amoils, C Heney
Jun 27, 2007·Clinical Techniques in Small Animal Practice·Susette M Aquino
Nov 7, 2008·Veterinary Surgery : VS·Genilson Fernandes De QueirozMaria Lúcia Zaidan Dagli
Feb 17, 2009·Investigative Ophthalmology & Visual Science·Sangita P Patel, William M Bourne
Jan 26, 2010·Journal of Pharmacological and Toxicological Methods·Frank A Barile
Jun 11, 2010·Clinical Ophthalmology·Joo Youn OhWon Ryang Wee
Apr 2, 2011·Eye·R W ShultzJ S Pulido
May 18, 2011·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Menk K PrinsenM V W Wijnands
Aug 21, 2013·Veterinary Ophthalmology·Thierry Azoulay
Feb 11, 2014·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Elodie CazelleMarcel V W Wijnands
Jul 21, 2015·Veterinary Ophthalmology·Nadine NautscherWerner M Amselgruber

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