The effects of solutes on the freezing properties of and hydration forces in lipid lamellar phases

Biophysical Journal
Y H YoonJ Wolfe

Abstract

Quantitative deuterium nuclear magnetic resonance is used to study the freezing behavior of the water in phosphatidylcholine lamellar phases, and the effect upon it of dimethylsulfoxide (DMSO), sorbitol, sucrose, and trehalose. When sufficient solute is present, an isotropic phase of concentrated aqueous solution may coexist with the lamellar phase at freezing temperatures. We determine the composition of both unfrozen phases as a function of temperature by using the intensity of the calibrated free induction decay signal (FID). The presence of DMSO or sorbitol increases the hydration of the lamellar phase at all freezing temperatures studied, and the size of the increase in hydration is comparable to that expected from their purely osmotic effect. Sucrose and trehalose increase the hydration of the lamellar phase, but, at concentrations of several molal, the increase is less than that which their purely osmotic effect would be expected to produce. A possible explanation is that very high volume fractions of sucrose and trehalose disrupt the water structure and thus reduce the repulsive hydration interaction between membranes. Because of their osmotic effect, all of the solutes studied reduced the intramembrane mechanical stres...Continue Reading

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Citations

Jun 24, 2003·Cryobiology·Yonghyeon YoonJoe Wolfe
Nov 11, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Dong-Kuk LeeAyyalusamy Ramamoorthy
Mar 22, 2014·Journal of the Royal Society, Interface·Ben KentGary Bryant
Apr 16, 2014·Food Chemistry·Loïc Foucat, Marc Lahaye
Oct 18, 2015·Biochimica Et Biophysica Acta·Virginia MiguelAdriana B Pierini
Aug 1, 2015·Langmuir : the ACS Journal of Surfaces and Colloids·Ben KentChristopher J Garvey
Jul 21, 2004·Colloids and Surfaces. B, Biointerfaces·Gary Bryant, Karen L Koster
Oct 31, 2009·Journal of Controlled Release : Official Journal of the Controlled Release Society·Chengjun ChenXing Tang
Oct 25, 2000·Physical Review. E, Statistical Physics, Plasmas, Fluids, and Related Interdisciplinary Topics·J Pencer, F R Hallett
Feb 5, 2009·The Journal of Physical Chemistry. B·Thomas LennéGary Bryant
Nov 13, 2019·Langmuir : the ACS Journal of Surfaces and Colloids·Sławomir S StachuraRicardo L Mancera
Feb 27, 2020·The Journal of Physical Chemistry Letters·Niki BaccileFrancisco M Fernandes

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