PMID: 9171162May 1, 1997Paper

The effects of storage conditions and trophic supplementation on the survival of fetal mesencephalic cells

Cell Transplantation
P ThajebP M Carvey

Abstract

It is estimated that only 5-10% of dopamine (DA) neurons implanted into the striatum of patients undergoing fetal-nigral transplantation as a treatment for Parkinson's Disease survive. Because it is often necessary to store fetal tissue prior to transplantation, we evaluated various storage parameters that could influence DA neuron viability in rostral mesencephalic tegmentum (RMT) cultures using tyrosine hydroxylase immunoreactive (THir) cell counts as an index of DA neuron survival. A high K+ hibernation media (HM) was used in all studies. We found that RMT cell viability and THir cell counts decreased as storage duration increased (up to 120 h). Storage at 37 degrees C in HM killed all cells, while storage at 10 degrees C yielded higher survival rates than 4 degrees C. In comparison to trypsinization, mechanical dissociation of tissue increased cell viability. Neutral pH and a storage density of at least 1 x 10(6) cells/mL were found to be optimal, while striatal coculture of RMT cells with striatal feeder layers increased THir viability up to 16-fold in comparison to monocultures. The nurturing effect of striatal coculture may be explained by the release of autotrophic factors, and we tested this hypothesis by supplementing...Continue Reading

Citations

Feb 5, 2002·Expert Opinion on Pharmacotherapy·C B Hurelbrink, R A Barker
Dec 25, 2003·Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]·Walter KruglugerJoerg Hugeneck
Dec 5, 2003·Acta Neurologica Scandinavica·R Tinsley, P Eriksson
May 9, 2006·Journal of Clinical Neuroscience : Official Journal of the Neurosurgical Society of Australasia·Peterus ThajebShinn-Zong Lin

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