The effects of streptozotocin-induced diabetes on ghrelin expression in rat testis: biochemical and immunohistochemical study

Folia Histochemica Et Cytobiologica
Mehmet F SönmezMunis Dundar

Abstract

Ghrelin is a hormone which has effects on the secretion of growth hormone, gastrointestinal system, cardiovascular system, cell proliferation and reproductive system. The present study we focused on the relation between ghrelin and GHS-R1a gene expression and the regulation of their expression in the testes of diabetic rats. 40 male Wistar albino rats were divided into four groups: control, and sampled 4, 8 and 12 weeks after induction of diabetes by streptozotocin (STZ) intraperitoneal injection (40 mg/kg). The rats were decapitated under ketamine anesthesia and their testes were removed. Blood was obtained from heart and serum follicle stimulating hormone (FSH), luteinizing hormone (LH), and testosterone levels were measured by ELISA. Tissue ghrelin and GHS-R mRNA levels were determined by qRT-PCR, while ghrelin protein expression was studied by immunohistochemistry. Histopathological damage scores were also assessed. Eight weeks after diabetes induction serum FSH level was increased, whereas LH and testosterone concentrations decreased. The ghrelin and GHS-R1a gene expression and ghrelin immunohistochemistry score first tended to increase after first four weeks of diabetes, and then tended to decrease. Ghrelin-immunopositive...Continue Reading

Citations

Nov 15, 2015·Systems Biology in Reproductive Medicine·Mehmet Fatih SönmezMunis Dündar
Sep 3, 2016·Experimental and Molecular Pathology·Kübra Tuğçe ÇilenkMehmet Fatih Sönmez
Mar 16, 2017·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·M F SönmezE Kaymak
Aug 10, 2021·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·Derya KarabulutBirkan Yakan

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cardiovascular Homeostasis

Cardiovascular regulation and homeostasis is important in maintaining health and dysfunction may lead to cardiovascular diseases. Nitric oxide and vascular endothelium are one of many molecules involved in the homeostatic mechanism. Here is the latest research on cardiovascular homeostasis.

Cardiovascular Disorder in Diabetes

Diabetes is associated with an increased risk of cardiovascular disorders and heart failure. Discover the latest research here.

© 2021 Meta ULC. All rights reserved