The effects of synthetic alpha-subunit peptides on thyrotropin interaction with its receptor

Endocrinology
J C MorrisR J Ryan

Abstract

Synthetic peptides of the alpha-subunit of human glycoprotein hormones have been shown previously to inhibit binding of [125I]iodo-hCG to ovarian membranes, thus indicating the importance of the alpha-subunit in the structure-function relationships of the gonadotropic hormone. These same synthetic alpha-subunit peptides, the sequences of which are common to all human glycoprotein hormones, were found to inhibit the binding of [125I]iodo-TSH to human thyroid membrane preparations and FRTL-5 rat thyroid cells. The active portions of the subunit were represented in synthetic peptides alpha 21-35, alpha 31-45, alpha 26-46, and alpha 81-92, indicating that 2 separate sites within the alpha-subunit have binding activity for TSH. Peptides alpha 26-46 and alpha 31-45 were also found to potently inhibit the stimulation of adenylate cyclase activity by bovine TSH in TSH bioassay using FRTL-5 cells. Seven other synthetic peptides, including the remainder of the 92-amino acid sequence of the alpha-subunit, demonstrated little or no ability to inhibit binding of the tracer or inhibit the bioactivity of intact TSH. The findings were very similar to those of previous studies involving hCG binding, except that the two active sites appeared to ...Continue Reading

Citations

Oct 1, 1990·Molecular and Cellular Endocrinology·M J PapandreouC Ronin
Jan 22, 1990·Molecular and Cellular Endocrinology·R SalesseJ Garnier
Jul 30, 1990·Molecular and Cellular Endocrinology·U E Petäjä-Repo, H J Rajaniemi
May 1, 1992·Molecular and Cellular Endocrinology·R S Weiner, J A Dias
Jul 1, 1992·Molecular and Cellular Endocrinology·H T Keutmann
Oct 1, 1996·Trends in Endocrinology and Metabolism : TEM·M W SzkudlinskiB D Weintraub
Oct 1, 1989·Journal of Steroid Biochemistry·M RogerR Scholler

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