PMID: 701367Oct 1, 1978Paper

The effects of temperature and glucose on protein biosynthesis by immature (round) spermatids from rat testes

The Journal of Cell Biology
M NakamuraP F Hall

Abstract

A method is described for the preparation of highly purified fractions (greater than 80% pure) of immature spermatids (round, steps 1--8) from rat testes by centrifugal elutriation in sufficient yields for biochemical studies when four rat testes are used. Electron microscopy established the identity of the cells and demonstrated that the cell membrane is intact. Some cells develop nuclear and cytoplasmic vacuoles during the 2 h required for preparation. Immature spermatids prepared by this method use glucose with an increase in oxygen consumption, lactate production, and protein synthesis over control levels (no glucose). The testicular cell suspension from which spermatids are separated, like whole testis and spermatids themselves, show higher incorporation of amino acids into TCA-precipitable material at 34 degrees C than at 38 degrees C and in the presence of glucose. A subcellular system prepared from immature spermatids with excess ATP shows greater incorporation of amino acids into TCA-precipitable material at 34 degrees C than at 38 degrees C. This difference does not result from increased breakdown of protein. It is concluded that body temperature (38 degrees C) inhibits some aspect(s) of protein synthesis in addition ...Continue Reading

References

May 1, 1977·Journal of Reproduction and Fertility·W J Barcellona, M L Meistrich
Apr 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·M Shikita, P F Hall
Feb 1, 1969·Endocrinology·A R Means, P F Hall
Nov 1, 1967·Endocrinology·A R Means, P F Hall

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Citations

Mar 6, 2007·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Takuya ChibaKeiichi Higuchi
Feb 1, 1984·The Journal of Cell Biology·G L Gerton, C F Millette
Sep 26, 2012·Cellular and Molecular Life Sciences : CMLS·Byunghyuk KimKunsoo Rhee
Jan 1, 1987·Archives of Andrology·V SantiemmaA Fabbrini
Jan 1, 1989·Archives of Andrology·M FujisawaS Kamidono
Oct 2, 2004·Andrologia·M MovahedinA Kazemnejad
Sep 29, 2011·Molecular Reproduction and Development·Tushna DehnugaraM R Satyanarayana Rao
May 4, 2005·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Kaan AydosCihat Unlü
Sep 28, 2018·Stem Cell Research & Therapy·Keykavos GholamiMehdi Abbasi
Mar 1, 1995·Andrologia·S C RubensteinA Morgentaler

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