The effects of temperature shock on transcription and replication in Rhynchosciara americana (Diptera: Sciaridae)

Genetica
A J StockerE Gorab

Abstract

The chromosomal response to temperature shock in Rhynchosciara americana is similar to that observed in other Diptera. After a 33 degrees C/90 min or a 36 degrees C/30 min shock the reaction for RNA polymerase II (RpII) is enhanced at five loci. The most prominent of these was identified by in situ hybridization as the site of the hsp70 gene. At 33 degrees C, an accumulation of heat shock factor (HSF) and an increase in the level of RpII was observed at some heat shock loci after 5 min and reached a maximum after 15 min at most loci. The pattern of accumulation of HSF and RpII at individual heat shock loci was similar and their increases were generally coordinated among the loci. RpII gradually decreased at sites active prior to shock, the rate of decrease varying with the site. The B2 DNA puff retained RpII for a significant length of time while the histone locus still contained RpII after a shock of 90 min. With a 36 degrees C/30 min shock, the size of the heat shock puffs and the intensities of HSF and RpII peaked at 1-4 h post stress. The level of HSF declined rapidly after 1 h while the level of RpII remained high for an additional 4 h. The reaction of the DNA puffs to heat shock varied. Usually they did not regress comple...Continue Reading

References

Jan 1, 1981·Chromosoma·G MorcilloJ L Díez
Mar 1, 1994·Experimental Cell Research·G MorcilloL M Botella
Nov 20, 2001·Nucleic Acids Research·J L MartinezJ L Diez

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Citations

Sep 22, 2009·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Alexandre de AndradeGlaucia Maria Machado-Santelli
Oct 23, 2015·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Alejandra Badaracco, Eduardo Gorab
Oct 5, 2006·Evolution; International Journal of Organic Evolution·A D Johansen-Morris, R G Latta

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