PMID: 9160162Apr 1, 1997Paper

The effects of temperature upon calcium exchange in intact cultured cardiac myocytes

Cell Calcium
F D MarengoG A Langer

Abstract

Cardiac myocyte Ca transport systems, such as sarcoplasmic reticulum Ca-ATPase and sarcolemmal Na/Ca exchange (Na/Ca), are critically dependent on temperature. The purpose of this work was to study the effect of temperature on cellular Ca compartmentation and its exchange characteristics in intact functional neonatal cultured myocytes. The Na/Ca mediated Ca exchange (CaNa/Ca)--including its sarcoplasmic reticulum (SR) and sarcolemmal (SL) contributions, a slow exchange component related to mitochondrial Ca and the La displaceable Ca pool were studied combining isotopic and gas-dissection techniques for membrane isolation. The major findings of this study are: (i) The amount of Ca exchanged through CaNa/Ca is clearly dependent on temperature (Q10 approximately 1.6) in the range studied (17-37 degrees C); (ii) the addition of 1 microM nifedipine does not modify the temperature dependence of CaNa/Ca; (iii) the sarcolemmal bound fraction contributing to CaNa/Ca is not changed by temperature; (iv) the increase in CaNa/Ca with temperature is explained by an increment in the contribution of SR-Ca to CaNa/Ca; (v) a fraction of SR which does not exchange via CaNa/Ca at low temperatures can be released and mobilized by caffeine-this caff...Continue Reading

References

Sep 14, 1978·Biochemical and Biophysical Research Communications·A J Williams, S E Barrie
Jul 1, 1992·The Journal of Membrane Biology·J A Post, G A Langer
Feb 11, 1991·Biochimica Et Biophysica Acta·M M BersohnK D Philipson
Oct 30, 1985·Biochimica Et Biophysica Acta·D Harding, C Fry
Nov 1, 1989·Journal of Molecular and Cellular Cardiology·J H Kuwata, G A Langer
Jan 1, 1995·The American Journal of Physiology·G A LangerT L Rich
Feb 1, 1963·Experimental Cell Research·I HARARY, B FARLEY
Feb 1, 1963·Experimental Cell Research·I HARARY, B FARLEY

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Citations

May 23, 2017·PloS One·Olga V NakipovaAlexey E Alekseev
Oct 18, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Steven E AndersonPeter M Cala

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