PMID: 15227740Jul 1, 2004Paper

The effects of Treponema pallidum on human dendritic cells

Yonsei Medical Journal
Jong Lan ShinMin Geol Lee

Abstract

Cell mediated immune responses play a prominent role in syphilis, which is caused by Treponema pallidum. The role of dendritic cells (DC) in the syphilitic infection is not well understood in human. In the present study, we studied interaction of T. pallidum with DC, generated from human peripheral blood mononuclear cells with GM-CSF and IL-4. After adding T. pallidum for 16 hours to immature DC at culture day 7, the change of surface antigens on DC was monitored by flow cytometry, the amount of IL-12 in culture supernatant of DC was measured by ELISA and T cell stimulatory capacity of DC was checked in mixed lymphocyte reaction (MLR). We have observed an efficient phagocytosis of T. pallidum by electron microscopy as early as 2 hours after addition of T. pallidum to DC. Interaction of DC with T. pallidum resulted in increased surface expression of CD83 which was proportionally increased according to the number of T. pallidum. Expressions of CD80, CD86 and HLA-DR on DC were slightly increased. The amount of IL-12 in the culture supernatant of DC was increased (1,099 pg/ml) after the addition of T. pallidum. T. pallidum-infected DC also displayed enhanced T cell stimulatory capacity in MLR. As seen from the above, we observed ph...Continue Reading

Citations

Jan 15, 2009·Folia Histochemica Et Cytobiologica·Anna Michalak-StomaMałgorzata Bartkowiak-Emeryk
Mar 18, 2011·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Hong-Wei JiangFu-Ren Zhang
Sep 17, 2005·AIDS Patient Care and STDs·Sergio SabbataniFrancesco Chiodo
Nov 27, 2008·The American Journal of Surgical Pathology·Fabio FacchettiPier Luigi Chiodera
Dec 3, 2011·The Journal of Clinical Investigation·Emily L Ho, Sheila A Lukehart
Jan 19, 2006·Clinical Microbiology Reviews·Rebecca E Lafond, Sheila A Lukehart

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