The effects of tunicamycin on the metabolism of acetylated low density lipoproteins

Cell Biochemistry and Function
D P Armstrong, D A White

Abstract

The effect of tunicamycin (TM) on the metabolism of acetylated low-density lipoprotein (AcLDL) was examined to determine whether N-linked glycosylation is required for the proper function of the AcLDL pathway. Proteolytic degradation of [125I]-AcLDL was increased twofold in the presence of TM. This did not occur via an increase in total lysosomal enzyme activity or extracellular proteolysis; rather, the rate of uptake of [125I]-AcLDL was increased. The enhanced degradation of AcLDL did not lead to a commensurate increase in the rate of synthesis of cholesteryl oleate. Conversely, the rate of cholesterol esterification was reduced in the presence of TM. The uptake of [125I]-AcLDL was more sensitive to inhibition by chloroquine in TM-treated cells. However, the presence of TM did not affect the ability of chloroquine to inhibit constitutive recycling of AcLDL binding sites. These results suggest that N-linked glycosylation may be involved in the regulation of AcLDL metabolism in J774 cells.

References

Jan 2, 1976·Clinica Chimica Acta; International Journal of Clinical Chemistry·J ShepherdH G Morgan
Sep 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·S K BasuM S Brown
Jan 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·J L GoldsteinM S Brown
Feb 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·W PalinskiJ L Witztum
Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·T KodamaM Krieger
Jan 1, 1987·Annual Review of Biochemistry·A D Elbein
Jan 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·S ParthasarathyD Steinberg
Jan 1, 1987·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·M E RosenfeldR Ross
Aug 1, 1985·Infection and Immunity·K W HranitzkyL Eidels
Jan 1, 1983·Annual Review of Biochemistry·M S Brown, J L Goldstein
Sep 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·J M Prives, K Olden
Sep 1, 1955·The Journal of Clinical Investigation·R J HAVELJ H BRAGDON

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antimalarial Agents (ASM)

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.

ApoE, Lipids & Cholesterol

Serum cholesterol, triglycerides, apolipoprotein B (APOB)-containing lipoproteins (very low-density lipoprotein (VLDL), immediate-density lipoprotein (IDL), and low-density lipoprotein (LDL), lipoprotein A (LPA)) and the total cholesterol/high-density lipoprotein (HDL) cholesterol ratio are all connected in diseases. Here is the latest research.

Antimalarial Agents

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.